The notable relatedness between ESBL producing Enterobacteriaceae isolated from clinical samples and asymptomatic fecal carriers

• Published in: BMC infectious diseases Vol. 23; no. 1; pp. 1 - 775
• Main Authors: Aghamohammad, Shadi, Shahcheraghi, Fereshteh
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 08.11.2023; BioMed Central; BMC
• Subjects: Antibiotics; Antimicrobial agents; Antimicrobial resistance; Asymptomatic; Bacteria; Bacterial pneumonia; Beta lactamases; Biofilms; Clinical isolates; Clinical sources; Clonal relatedness; Clusters; Comparative analysis; Drug resistance; Drug resistance in microorganisms; E coli; Enterobacteriaceae; Fecal carriage; Feces; Genes; Imipenem; Infections; Patients; Pneumonia; Reservoirs; Risk factors; Software; Urine; Virulence; Virulence (Microbiology); Virulence factors; β Lactamase; Iran
• ISSN: 1471-2334; 1471-2334
• DOI: 10.1186/s12879-023-08746-3

The investigation of the presence of extended-spectrum beta-lactamase (ESBL) within Enterobacteriaceae in both fecal carriers and patients is an essential matter. Furthermore, the assessment of distinct characteristics exhibited by resistant bacteria obtained from fecal carriers and patients, as well as the comparison of these characteristics between the two groups, could provide a deeper understanding of how the resistant isolates can remain concealed within a dormant reservoir and intensify antimicrobial resistance. The aim of the present study was to concentrate on the comparison of the antimicrobial resistance pattern and molecular features between strains obtained from clinical and carrier sources. A total of 142 clinical samples and 120 rectal swabs were collected from June to October 2016. ESBL screening was performed using the double-disk synergy test. PCR was done for the detection of ESBL genes. Assessment of biofilm formation, virulence factor genes, and MLVA was performed for K. pneumonae isolates. Phylogroup typing was performed for E. coli isolates. Of 146 samples, 67.6% were E. coli, and 32.4% were K. pneumoniae. The rate of bla.sub.CTXM-15 was 89.4%. In K. pneumoniae type D, ompk35 and fimH were the highest. All the K. pneumoniae isolates were classified into 12 mini clusters and the clinical isolates were characterized into 7 mini clusters. The phylogroup B2 in ESBL-EC was the highest (56.2%). Comparison of molecular characteristics and clonal relatedness between fecal carriers and patients showed noticeable relatedness and similarity which may indicate that ESBL-KP can be colonized with the same profiles in different settings and, therefore, may be widely distributed in both community and hospital settings. Therefore, implementation of control protocols, including surveillance of the fecal carriers, could impressively reduce silent reservoirs without clinical symptoms as well as patient rates.