HDAC inhibitor increases AcMNPV gene expression in mammalian cells

• Published in: Archives of virology Vol. 155; no. 4; pp. 577 - 581
• Main Authors: Fujita, Ryosuke, Ohtsuka, Daisuke, Sahara, Ken, Asano, Shinichiro, Bando, Hisanori
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01.04.2010; Springer Vienna; Springer; Springer Nature B.V
• Subjects: Animals; Autographa californica; Baculovirus; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Brief Report; Cell cycle; Cell Line; Cricetinae; DNA methylation; Epigenetics; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Viral; Gene therapy; Genetics; Histone Deacetylase Inhibitors - metabolism; Host-Pathogen Interactions; Immunoprecipitation; Infections; Infectious Diseases; Medical Microbiology; Mesocricetus; Microbiology; Miscellaneous; Nuclear polyhedrosis virus; Nucleopolyhedrovirus - physiology; Polymerase Chain Reaction; Proteins; Vectors (Biology); Virology; Viruses; MNPV; Histone deacetylation; Epigenetic; Baculovirus; Gene therapy; Nucleosome; Gene expression; Vertebrata; Mammalia
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-010-0614-3

The baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is used as a safer viral vector in mammalian cells with potential applications in gene therapy. However, the mechanism for the insusceptibility of mammalian cells to proliferative infection by entomopathogenic viruses is not well understood. Here, we studied the significance of epigenetic modifications such as histone acetylation, histone methylation and HP1 accumulation for AcMNPV gene expression in mammalian BHK cells. Real-time PCR and chromatin immunoprecipitation with sodium butyrate revealed an important relationship between viral gene expression and histone acetylation, with implications for a mechanism of suppression of AcMNPV gene expression in BHK cells.