Improving the Detection Sensitivity of a New Rapid Diagnostic Technology for Severe Acute Respiratory Syndrome Coronavirus 2 Using a Trace Amount of Saliva

The early diagnosis and isolation of infected individuals with coronavirus disease 2019 (COVID-19) remain important. Although quantitative polymerase chain reaction (qPCR) testing is considered the most accurate test available for COVID-19 diagnosis, it has some limitations, such as the need for spe...

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Published inDiagnostics (Basel) Vol. 12; no. 11; p. 2568
Main Authors Tokuyama-Toda, Reiko, Terada-Ito, Chika, Muraoka, Masaaki, Horiuchi, Toshikatsu, Amemiya, Tsuyoshi, Fukuoka, Airi, Hamada, Yoshiki, Takebe, Yusuke, Ogawa, Takashi, Fujii, Seiko, Kikuta, Toshihiro, Sejima, Shunsuke, Satomura, Kazuhito
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 22.10.2022
MDPI
Subjects
Annealing
Communication
Coronaviruses
COVID-19
diagnostic technology
Disease control
Enzymes
Ethics
Gene amplification
Hospitals
Infections
Laboratories
Methods
mobile qPCR device
mouthwash
Pandemics
Polymerase chain reaction
saliva
Salivary diagnostics
SARS-CoV-2
Severe acute respiratory syndrome coronavirus 2
Japan
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Summary:The early diagnosis and isolation of infected individuals with coronavirus disease 2019 (COVID-19) remain important. Although quantitative polymerase chain reaction (qPCR) testing is considered the most accurate test available for COVID-19 diagnosis, it has some limitations, such as the need for specialized laboratory technicians and a long turnaround time. Therefore, we have established and reported a rapid diagnostic method using a small amount of saliva as a sample using a lightweight mobile qPCR device. This study aimed to improve the existing method and increase the detection sensitivity and specificity. The detection specificity of CDC N1 and N2 was examined by improving qPCR reagents and polymerase chain reaction conditions for the previously reported method. Furthermore, the feasibility of detecting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA was examined using both the previous method and the improved method in patients with COVID-19. The results showed that the improved method increased the specificity and sensitivity. This improved method is useful for the rapid diagnosis of SARS-CoV-2.
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ISSN:2075-4418
2075-4418
DOI:10.3390/diagnostics12112568