Effective Inhibition by Pentobarbital of Forskolin-Stimulated Adenylate Cyclase Activity in Rat Brain

• Published in: Chemical & pharmaceutical bulletin Vol. 37; no. 11; pp. 3142 - 3144
• Main Authors: DAN'URA, Toshio, KUROKAWA, Tomonori, YAMASHITA, Atsushi, ISHIBASHI, Sadahiko
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1989; Maruzen
• Subjects: adenylate cyclase; Adenylyl Cyclases - metabolism; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Brain - drug effects; Brain - enzymology; Colforsin - antagonists & inhibitors; Colforsin - pharmacology; Enzymes and enzyme inhibitors; forskolin; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; Lyases; Male; pentobarbital; Pentobarbital - pharmacology; rat brain; Rats; Rats, Inbred Strains; Synaptosomes - drug effects; Synaptosomes - enzymology; Brain; Rat; Rodentia; Enzyme inhibitor; Stimulation; Dose activity relation; Adenylate cyclase; Vertebrata; Synaptosome; Mammalia; Enzymatic activity; Forskolin; Plasma membrane; Barbituric
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.37.3142

The effect of pentobarbital on the adenylate cyclase system was examined in synaptosomal membranes from rat brain. Pentobarbital inhibited forskolin-stimulated enzyme activity more effectively than the basal and Mn2+ -stimulated enzyme activities. The degree of inhibition of the enzyme activity by pentobarbital was increased by the presence of forskolin in a concentration-dependent manner. No significant difference is observed in the degree of the inhibition by pentobarbital between the basal and forskolin-stimulated activities in the membranes prepared from the peripheral tissues.