Rre37 stimulates accumulation of 2-oxoglutarate and glycogen under nitrogen starvation in Synechocystis sp. PCC 6803

• Published in: FEBS letters Vol. 588; no. 3; pp. 466 - 471
• Main Authors: Joseph, Ancy, Aikawa, Shimpei, Sasaki, Kengo, Teramura, Hiroshi, Hasunuma, Tomohisa, Matsuda, Fumio, Osanai, Takashi, Hirai, Masami Yokota, Kondo, Akihiko
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 31.01.2014
• Subjects: 2-OG; 2-Oxoglutarate; Citric Acid Cycle; Cyanobacteria; DNA-Binding Proteins; fructose 6-phosphate; Fructosephosphates - metabolism; fumarates; Gene Expression Regulation, Bacterial; glutamate synthase; Glutamate Synthase - metabolism; glutamic acid; glutamine synthetase; glyceraldehyde 3-phosphate; Glycogen; Glycogen - metabolism; Glycolysis; GOGAT; high performance liquid chromatography; HPLC; Ketoglutaric Acids - metabolism; LC/QqQ-MS; liquid chromatography/triple quadrupole mass spectrometry; metabolites; nitrogen; Nitrogen - metabolism; Nitrogen starvation; optical density; oxaloacetic acid; regulatory proteins; Rre37; Starvation; succinic acid; Synechocystis; Synechocystis - metabolism; TCA; tricarboxylic acid; tricarboxylic acid cycle; LC/QqQ-MS; Rre37; TCA; 2-OG; 2-Oxoglutarate; OD; Glycogen; Cyanobacteria; GS; Nitrogen starvation; HPLC; GOGAT; tricarboxylic acid; glutamate synthase; liquid chromatography/triple quadrupole mass spectrometry; high performance liquid chromatography; glutamine synthetase; optical density
• ISSN: 0014-5793; 1873-3468; 1873-3468
• DOI: 10.1016/j.febslet.2013.12.008

•Metabolomic analysis was applied on the Rre37 mutant of Synechocystis.•Role of Rre37 under nitrogen starvation was investigated.•2-Oxoglutarate accumulation was abolished in the Rre37 mutant.•Rre37 regulated glycogen accumulation through glycogen anabolism.•Rre37 regulated accumulation of TCA cycle metabolites. Rre37 (sll1330) in a cyanobacterium Synechocystis sp. PCC 6803 acts as a regulatory protein for sugar catabolic genes during nitrogen starvation. Low glycogen accumulation in Δrre37 was due to low expression of glycogen anabolic genes. In addition to low 2-oxoglutarate accumulation, normal upregulated expression of genes encoding glutamate synthases (gltD and gltB) as well as accumulation of metabolites in glycolysis (fructose-6-phosphate, fructose-1,6-bisphosphate, and glyceraldehyde-3-phosphate) and tricarboxylic acid (TCA) cycle (oxaloacetate, fumarate, succinate, and aconitate) were abolished by rre37 knockout. Rre37 regulates 2-oxoglutarate accumulation, glycogen accumulation through expression of glycogen anabolic genes, and TCA cycle metabolites accumulation.