Different Contributions of Microtubule Dynamics and Transport to the Growth of Axons and Collateral Sprouts

• Published in: The Journal of neuroscience Vol. 19; no. 10; pp. 3860 - 3873
• Main Authors: Gallo, Gianluca, Letourneau, Paul C
• Format: Journal Article
• Language: English
• Published: United States Soc Neuroscience 15.05.1999; Society for Neuroscience
• Subjects: Animals; Axons - physiology; Axons - ultrastructure; Biological Transport; Biopolymers; Cells, Cultured; Chick Embryo; Cytoskeleton - ultrastructure; Dendrites - physiology; Microtubules - physiology; Nocodazole - pharmacology; Paclitaxel - pharmacology
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/jneurosci.19-10-03860.1999

Axonal growth is believed to depend on microtubule transport and microtubule dynamic instability. We now report that the growth of axon collateral branches can occur independent of microtubule dynamic instability and can rely mostly on the transport of preassembled polymer. Raising embryonic sensory neurons in concentrations of either taxol or nocodazole (NOC) that largely inhibit microtubule dynamics significantly inhibited growth of main axonal shafts but had only minor effects on collateral branch growth. The collaterals of axons raised in taxol or nocodazole often contained single microtubules with both ends clearly visible within the collateral branch ("floating" microtubules), which we interpret as microtubules undergoing transport. Furthermore, in these collaterals there was a distoproximal gradient in microtubule mass, indicating the distal accumulation of transported polymer. Treatment of cultures with a high dose of nocodazole to deplete microtubules from collaterals, followed by treatment with 4-20 nM vinblastine to inhibit microtubule repolymerization, resulted in the time-dependent reappearance and subsequent distal accumulation of floating microtubules in collaterals, providing further evidence for microtubule transport into collateral branches. Our data show that, surprisingly, the contribution of microtubule dynamics to collateral branch growth is minor compared with the important role of microtubule dynamics in growth cone migration, and they indicate that the transport of microtubules may provide sufficient cytoskeletal material for the initial growth of collateral branches.