Active drug encapsulation and release kinetics from hydrogel-in-liposome nanoparticles

• Published in: Journal of colloid and interface science Vol. 406; pp. 247 - 255
• Main Authors: Wang, Yan, Tu, Sheng, Pinchuk, Anatoly N., Xiong, May P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 15.09.2013; Elsevier
• Subjects: Activation; Active; Active control; antineoplastic agents; Antineoplastic Agents - administration & dosage; Antineoplastic Agents - pharmacokinetics; Benzoquinones - administration & dosage; Benzoquinones - pharmacokinetics; cell culture; Chemistry; Colloidal gels. Colloidal sols; Colloidal state and disperse state; Cytotoxicity; Drug delivery; Drug Delivery Systems; Drug loading; Drugs; electrostatic interactions; Encapsulation; Exact sciences and technology; Female; Geldanamycin; gels; General and physical chemistry; Humans; hydrocolloids; Hydrogel; Hydrogels; Lactams, Macrocyclic - administration & dosage; Lactams, Macrocyclic - pharmacokinetics; lipid bilayers; Liposome; Liposomes - administration & dosage; Male; Membranes; Nanogel; Nanoparticles; Nanoparticles - administration & dosage; Passive; Physical and chemical studies. Granulometry. Electrokinetic phenomena; Polymerization; Release kinetics; solubility; temperature; Tumor Cells, Cultured; Active; Nanogel; Passive; Release kinetics; Liposome; Cytotoxicity; Geldanamycin; Drug delivery; Hydrogel; Drug loading; Encapsulation; Drug; Nanoparticle; Drug carrier; Colloidal gel; Kinetics; Release
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2013.05.081

[Display omitted] •Hydrogel-in-liposomes (lipogels) were prepared by UV irradiation.•The model drug used was 17-DMAPG, a geldanamycin derivative.•Passive and active drug loading, and release properties were characterized.•The in vitro cytotoxicity of the nanoparticles and materials were investigated. Herein, we demonstrate for the first time the use of hydrogel-in-liposome nanoparticles (lipogels) as a promising drug delivery vehicle for the active encapsulation of the anticancer drug 17-DMAPG, a geldanamycin (GA) derivative. This model drug was chosen due to its improved aqueous solubility (4.6mg/ml) compared to the parent GA (<0.01mg/ml), and presence of a tertiary amine which readily protonates at low pH. For the design of lipogels, a PAA hydrogel core was formed inside liposomes through UV-initiated DEAP activation and polymerization of AA and BA. We have demonstrated here that electrostatic interactions between drug and gel are critical for active encapsulation and sustained release of 17-DMAPG. We found that optimal loading conditions could be obtained (88% loading efficiency) through control of pH, temperature and incubation time. Dramatic sustained drug release from lipogels was achieved independent of the external solution pH (ca. 54h to 50% drug release) and confirmed that the lipid bilayer was intact in the presence of the gel core. In vitro cell culture studies revealed that at the highest concentration tested, which corresponded to approximately 0.4mg/ml of material, lipogels did not exert cytotoxicity to cells.