Fusobacterium Detected in Colonic Biopsy and Clinicopathological Features of Ulcerative Colitis in Japan
Background and Aims Fusobacterium species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. Here, we performed prevalence screening of Fusobacterium in ulcerative colit...
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Published in | Digestive diseases and sciences Vol. 60; no. 1; pp. 205 - 210 |
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Main Authors | , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Boston
Springer US
01.01.2015
Springer Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Background and Aims
Fusobacterium
species are part of the gut microbiome in humans, but some species have been recognized as opportunistic pathogens implicated in inflammatory diseases including inflammatory bowel diseases. Here, we performed prevalence screening of
Fusobacterium
in ulcerative colitis (UC) in Japanese patients.
Methods
We examined
Fusobacterium nucleatum
(
F. nucleatum
) and whole
Fusobacterium
species
(
Pan
-
fusobacterium
) by quantitative real-time PCR in 163 inflamed mucosae from 152 UC patients. Data were correlated with clinical subtypes of UC.
Results
In an initial prevalence screen,
F. nucleatum
and
Pan
-
fusobacterium
were detected in 6.3 % (4/64) and 53.1 % (34/64). For all 163 mucosae, the prevalence of
Pan
-
fusobacterium
was 54.6 % (89/163).
Pan
-
fusobacterium
status was concordant in inflamed and normal adjacent samples, and the matched cases during 1-year follow-up colonoscopy. The higher amount of
Pan
-
fusobacterium
was observed in chronic continuous type compared to one attack and relapse/remitting type (
p
= 0.039). The higher amount of
Pan
-
fusobacterium
was also associated with rather mild clinical course of disease, such as non-steroid dependency (
p
= 0.015), non-refractory phenotype (
p
= 0.013), and non-severe phenotype (
p
= 0.04). Based on the distribution of
Pan
-
fusobacterium
measurable cases, we identified 10 cases as having a high amount of
Pan
-
fusobacterium
(FB-high). The clinicopathological features of FB-high UC cases were also highlighted by chronic continuous type and mild phenotypes of disease.
Conclusion
Whole
Fusobacterium
species, but not
F. nucleatum
, are common in UC patients and have a role in persistence of colonic inflammation in UC. However,
Fusobacterium
infection is associated with rather mild clinical phenotypes of UC. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0163-2116 1573-2568 |
DOI: | 10.1007/s10620-014-3316-y |