Four-Dimensional Migratory Coordinates of GABAergic Interneurons in the Developing Mouse Cortex

We have used time-lapse multiphoton microscopy to map the migration and settling pattern of GABAergic interneurons that originate in the ganglionic eminence of the ventral forebrain and incorporate into the neocortex of the cerebral hemispheres. Imaging of the surface of the cerebral hemispheres in...

Full description

Saved in:
Bibliographic Details
Published inThe Journal of neuroscience Vol. 23; no. 13; pp. 5805 - 5815
Main Authors Ang, Eugenius S. B. C., Jr, Haydar, Tarik F, Gluncic, Vicko, Rakic, Pasko
Format Journal Article
LanguageEnglish
Published United States Soc Neuroscience 02.07.2003
Society for Neuroscience
Subjects
Animals
Bromodeoxyuridine - pharmacokinetics
Calbindins
Cell Movement - physiology
Cerebral Cortex - cytology
Cerebral Cortex - embryology
Development/Plasticity/Repair
gamma-Aminobutyric Acid - metabolism
Immunohistochemistry
In Vitro Techniques
Interneurons - cytology
Interneurons - metabolism
Mice
Microscopy, Video - methods
S100 Calcium Binding Protein G - biosynthesis
Time Factors
Online AccessGet full text

Cover

More Information
Summary:We have used time-lapse multiphoton microscopy to map the migration and settling pattern of GABAergic interneurons that originate in the ganglionic eminence of the ventral forebrain and incorporate into the neocortex of the cerebral hemispheres. Imaging of the surface of the cerebral hemispheres in both explant cultures and brains of living mouse embryos revealed that GABAergic interneurons migrating within the marginal zone originate from three different sources and migrate via distinct and independent streams. After reaching their areal destination, interneurons descend into the underlying cortex to assume positions with isochronically generated, radially derived neurons. The dynamics and pattern of cell migration in the marginal zone (see movies, available at www.jneurosci.org) suggest that the three populations of interneurons respond selectively to distinct local cues for directing their migration to the appropriate areas and layers of the neocortex. This approach opens a new avenue for study of normal and abnormal neuronal migration in their native environment and indicate that interneurons have specific programs for their areal and laminar deployment.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0270-6474
1529-2401
DOI:10.1523/jneurosci.23-13-05805.2003