Monoclonal neutralizing antibodies against EV71 screened from mice immunized with yeast-produced virus-like particles

• Published in: Virologica Sinica Vol. 30; no. 3; pp. 208 - 213
• Main Authors: Lin, Tao, Xianyu, Lingzhi, Lyu, Songya
• Format: Journal Article
• Language: English
• Published: Wuhan Wuhan Institute of Virology, CAS 01.06.2015; KeAi Publishing Communications Ltd
• Subjects: 71(EV71); and; Animals; antibodies; Antibodies, Neutralizing - immunology; Antibodies, Neutralizing - isolation & purification; Antibodies, Viral - immunology; Antibodies, Viral - isolation & purification; Biochemistry; Biomedical and Life Sciences; Biomedicine; Capsid protein; Capsid Proteins - immunology; children; China; coat proteins; death; diagnostic techniques; disease; Drug development; enterovirus; Enterovirus A; Enterovirus A, Human - immunology; Female; females; hand,foot; Hand-foot-and-mouth disease; hybridoma; hybridomas; immunity; Immunization, Passive - methods; Immunoglobulins; Medical Microbiology; mice; Mice, Inbred BALB C; Microbial Genetics and Genomics; Microbiology; Monoclonal antibodies; mouth; neutralization; neutralizing; neutralizing antibodies; Oncology; particles(VLPs); pathogens; Research Article; Saccharomyces cerevisiae - genetics; Serologic Tests - methods; Vaccines, Synthetic - administration & dosage; Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology; Vaccines, Synthetic - isolation & purification; Vaccines, Virus-Like Particle - administration & dosage; Vaccines, Virus-Like Particle - genetics; Vaccines, Virus-Like Particle - immunology; Vaccines, Virus-Like Particle - isolation & purification; viral vaccines; Virology; virus-like; Virus-like particles; VP1 protein; yeasts; China; virus-like particles (VLPs); hand, foot and mouth disease; enterovirus 71 (EV71); neutralizing antibodies; hybridoma
• ISSN: 1674-0769; 1995-820X
• DOI: 10.1007/s12250-015-3573-8

Periodic outbreaks of hand, foot and mouth disease (HFMD) occur in children under 5 years old, and can cause death in some cases. The C4 strain of enterovirus 71 (EV71) is the main pathogen that causes HFMD in China. Although no drugs against EV71 are available, some studies have shown that candidate vaccines or viral capsid proteins can produce anti-EV71 immunity. In this study, female BABL/c mice (6–8 weeks old) were immunized with virus-like particles (VLPs) of EV71 produced in yeast to screen for anti-EV71 antibodies. Two hybridomas that could produce neutralizing antibodies against EV71 were obtained. Both neutralizing mAbs (D4 and G12) were confirmed to bind the VP1 capsid protein of EV71, and could protect >95% cells from 100 TCID50 EV71 infection at 25 µg/mL solution (lowest concentration). Those two neutralizing mAbs identified in the study may be promising candidates in development for mAbs to treat EV71 infection, and utilized as suitable reagents for use in diagnostic tests and biological studies.