Bletilla striata polysaccharides ameliorates lipopolysaccharide-induced injury in intestinal epithelial cells

• Published in: Saudi journal of gastroenterology Vol. 25; no. 5; pp. 302 - 308
• Main Authors: Luo, Lei, Liu, Yuqing, Cai, Xin, Wang, Yao, Xue, Juan, Zhang, Juan, Yang, Fan
• Format: Journal Article
• Language: English
• Published: India Wolters Kluwer India Pvt. Ltd 01.09.2019; Medknow Publications and Media Pvt. Ltd; Medknow Publications & Media Pvt. Ltd; Wolters Kluwer - Medknow; Wolters Kluwer Medknow Publications
• Subjects: Animals; Biotechnology; Bletilla striata polysaccharide; Blotting, Western; Care and treatment; Cattle; Cell Line; Cell Membrane Permeability - drug effects; Chemical properties; Cytokines; Cytokines - metabolism; Disease Models, Animal; Enteritis - drug therapy; Enteritis - metabolism; Enteritis - pathology; Enzyme-Linked Immunosorbent Assay; Epithelial Cells - drug effects; Epithelial Cells - pathology; Experiments; Health aspects; Humidity; IEC-18 cells; Intestinal diseases; Intestinal mucosa; Intestinal Mucosa - drug effects; Intestinal Mucosa - pathology; Intestine, Small - drug effects; Intestine, Small - metabolism; Intestine, Small - pathology; Lipopolysaccharides - toxicity; occludin; Orchids; Original; Permeability; Physiological aspects; Phytochemicals; Polysaccharides; Polysaccharides - pharmacology; Proteins; Rabbits; Rats; Studies; Tumor necrosis factor-TNF; ZO-1; China; United States > US; occludin; ZO-1; Bletilla striata polysaccharide; IEC-18 cells
• ISSN: 1319-3767; 1998-4049
• DOI: 10.4103/sjg.SJG_520_18

Background/Aims: This study was carried out to investigate the effect of Bletilla striata polysaccharide (BSP) treating on lipopolysaccharide (LPS)-induced intestinal epithelial barrier disruption in rat intestinal epithelial cell (IEC) line. Materials and Methods: LPS was used to stimulate the IEC-18 cells (1 μg/ml), with or without different concentrations of BSP (25, 50 and 100 μg/ml) for 24 h. Transepithelial electrical resistance (TEER) was measured to detect the permeability of cells. Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in the cell supernatant were detected with the enzyme-linked immunosorbent assay (ELISA). Real-time polymerase chain reaction (PCR) was employed to detect the mRNA levels of zonulae occludens (ZO)-1 and occludin. Western blot and immunofluorescence analysis were used for analyzing the expression level and the distribution patterns of ZO-1 and occludin protein. Results: After treatment with BSP, the IL-6 and TNF-α levels in the cell supernatant were significantly decreased compared with the experiment group (P < 0.05 or 0.01). The permeability of IEC was decreased in BSP groups when compared with the experiment group (P < 0.05 or 0.01). In addition, compared with the experiment group, treatment with BSP up-regulated mRNA and protein expression levels of ZO-1 and occludin, and kept the ZO-1 and occludin protein intact in IEC-18 cells injured with LPS (P < 0.05 or 0.01). Conclusion: BSP has the capacity to protect IEC-18 cells from LPS-induced injury. The mechanisms may be associated with decreasing the inflammatory cytokine levels of IL-6 and TNF-α, and elevating the expression of ZO-1 and occludin, which might serve as a new protective agent for LPS-induced intestinal epithelial barrier disruption.