MRP8/MRP14对宫颈癌细胞增殖的影响及机制

目的探讨髓样相关蛋白8/14异源二聚体（MRP8/MRP14）对宫颈癌细胞增殖的影响及机制。方法体外培养人宫颈癌Hela细胞,加入MRP8/MRP14（观察组）,另设对照组加入等体积PBS,于培养12、24、48、72 h分别采用CCK8法检测两组细胞增殖能力。取Hela细胞进行核质分离,加入MRP8/MRP14共培养30 min,采用Western blot法检测细胞凋亡相关信号通路蛋白p38 MAPK、JNK及细胞增殖相关信号通路蛋白ERK1/2、NF-κB表达。将Hela细胞分为MRP8/MRP14组和p38 MAPK、JNK、ERK1/2、NF-κB抑制剂组,RP8/MRP14组加入M...

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Bibliographic Details
Published in山东医药 Vol. 56; no. 22; pp. 24 - 26
Main Author 胡兵荣 王娟 罗海华 姜勇
Format Magazine Article
LanguageChinese
Published 南方医科大学基础医学院,广东省蛋白质组学重点实验室,广州510515 2016
Subjects
宫颈癌
细胞增殖
髓样相关蛋白8/14异源二聚体
髓样相关蛋白8/14异源二聚体
宫颈癌
cell proliferation
myeloid-related protein-8/14 heterodimer
cervical carcinoma
细胞增殖
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ISSN1002-266X
DOI10.3969/j.issn.1002-266X.2016.22.008

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Summary:目的探讨髓样相关蛋白8/14异源二聚体（MRP8/MRP14）对宫颈癌细胞增殖的影响及机制。方法体外培养人宫颈癌Hela细胞,加入MRP8/MRP14（观察组）,另设对照组加入等体积PBS,于培养12、24、48、72 h分别采用CCK8法检测两组细胞增殖能力。取Hela细胞进行核质分离,加入MRP8/MRP14共培养30 min,采用Western blot法检测细胞凋亡相关信号通路蛋白p38 MAPK、JNK及细胞增殖相关信号通路蛋白ERK1/2、NF-κB表达。将Hela细胞分为MRP8/MRP14组和p38 MAPK、JNK、ERK1/2、NF-κB抑制剂组,RP8/MRP14组加入MRP8/MRP14;抑制剂组先分别加入p38 MAPK、JNK、ERK1/2、NF-κB抑制剂预处理2 h,再加入MRP8/MRP14;采用CCK8法观察各组细胞增殖能力。结果观察组加入MRP8/MRP14培养12、24、48、72 h后,细胞增殖能力均高于对照组,且随时间延长,细胞增殖能力逐渐增强（P均〈0.05）。观察组加入MRP8/MRP14培养30 min后,与对照组比较,p38 MAPK、JNK、ERK1/2活性明显升高,NF-κB在细胞核中的表达升高（P均〈0.05）。p38 MAPK、JNK抑制剂组细胞增殖能力较MRP8/MRP14组增强;ERK、NF-κB抑制剂组细胞增殖能力较MRP8/MRP14组减弱（P均〈0.05）。结论 MRP8/MRP14能够促进Hela细胞增殖,其机制与p38 MAPK、JNK、ERK1/2及NF-κB等多条相关信号通路被激活有关。
Bibliography:37-1156/R
HU Bingrong, WANG Juan, LUO Haihua, JIANG Yong （Southern Medical University, Guangzhou 510515, China）
Objective To investigate the effect of myeloid-related protein-8 /14 heterodimer（ MRP8 / MRP14） on the proliferation of cervical cancer Hela cells and the possible mechanism. Methods Cervical cancer cell line Hela was cultured in vitro and treated with MRP8 / MRP14（ observation group） or PBS（ control group）,then we measured the proliferation ability at 12,24,48 and 72 h with CCK8. The nuclear of Hela cells was separated and then Hela cells were cultured with MRP8 / MRP14 for 30 min. The expression of apoptosis-related signaling pathway protein p38 MAPK,JNK and proliferation-related signaling pathway protein ERK1 /2 and NF-κB was measured by Western blotting. Hela cells were divided into MRP8 / MRP14 group,p38 MAPK,JNK,ERK1 /2 and NF-κB inhibitor group. Hela cells in the MRP8 / MRP14 group were only treated with MRP8 / MRP14,and the inhibitor groups were pretreated with p38 MAPK,JNK,ERK1 /2 or NF-κB in
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2016.22.008