Simultaneous Multiplexed Detection of Protein and Metal Ions by a Colorimetric Microfluidic Paper-based Analytical Device

In order to improve the efficiency of disease diagnosis and environmental monitoring, it is desirable to detect the concentration of proteins and metal ions simultaneously, since the current popular diagnostic platform can only detect proteins or metal ions independently. In this work, we developed...

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Published inBiochip journal Vol. 14; no. 4; pp. 429 - 437
Main Authors Xiong, Xiaolu, Zhang, Junlin, Wang, Zhou, Liu, Chenchen, Xiao, Wende, Han, Junfeng, Shi, Qingfan
Format Journal Article
LanguageEnglish
Published Seoul The Korean Society for Applied Biological Chemistry 01.12.2020
Springer Nature B.V
한국바이오칩학회
Subjects
Biomedical Engineering and Bioengineering
Biotechnology
Bovine serum albumin
Calibration
Chemistry
Colorimetry
Diagnostic systems
Environmental monitoring
Iron
Metal ions
Microfluidics
Nickel
Original
Original Article
Proteins
Reagents
Serum albumin
Water sampling
생물공학
Ni(II)
BSA
Microfluidic paper-based analytical device
Fe(III)
Simultaneous
Colorimetric detection
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Summary:In order to improve the efficiency of disease diagnosis and environmental monitoring, it is desirable to detect the concentration of proteins and metal ions simultaneously, since the current popular diagnostic platform can only detect proteins or metal ions independently. In this work, we developed a colorimetric microfluidic paper-based analytical device (µPAD) for simultaneous determination of protein (bovine serum albumin, BSA) and metal ions [Fe(III) and Ni(II)]. The µPAD consisted of one central zone, ten reaction zones and ten detection zones in one device, in which reaction solutions were effectively optimized for different types of chromogenic reactions. Fe(III), Ni(II) and BSA can be easily identified by the colored products, and their concentrations are in good accordance with color depth based on the established standard curves. The detection limits are 0.1 mM for Fe(III), 0.5 mM for Ni(II) and 1µM for BSA, respectively. Best of all, we demonstrated the efficiency of the µPAD with accurate detection of Fe(III), Ni (II) and BSA from river water samples within 15 minutes. The µPAD detection is efficient, instrument-free, and easy-to-use, holding great potential for simultaneous detection of cross type analytes in numerous diagnostic fields.
Bibliography:https://link.springer.com/article/10.1007%2Fs13206-020-4407-9
ISSN:1976-0280
2092-7843
DOI:10.1007/s13206-020-4407-9