DNA/Ag Nanoparticles as Antibacterial Agents against Gram-Negative Bacteria

• Published in: Nanomaterials (Basel, Switzerland) Vol. 5; no. 1; pp. 284 - 297
• Main Authors: Takeshima, Tomomi, Tada, Yuya, Sakaguchi, Norihito, Watari, Fumio, Fugetsu, Bunshi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 03.03.2015; MDPI
• Subjects: antibacterial; Antibacterial agents; Antiinfectives and antibacterials; Bacteria; Cotton; Deoxyribonucleic acid; DNA; DNA/Ag nanoparticles; E coli; Efficiency; Electrons; electrostatic interaction; Escherichia coli; Fabrics; Gram-negative bacteria; Lattice theory; Nanomaterials; nanoparticle immobilization; Nanoparticles; Salmon; salmon milt DNA; Salmonidae; Silver; Toxicity; Japan; nanoparticle immobilization; electrostatic interaction; DNA/Ag nanoparticles; antibacterial; Gram-negative bacteria; salmon milt DNA
• ISSN: 2079-4991; 2079-4991
• DOI: 10.3390/nano5010284

Silver (Ag) nanoparticles were produced using DNA extracted from salmon milt as templates. Particles spherical in shape with an average diameter smaller than 10 nm were obtained. The nanoparticles consisted of Ag as the core with an outermost thin layer of DNA. The DNA/Ag hybrid nanoparticles were immobilized over the surface of cotton based fabrics and their antibacterial efficiency was evaluated using as the typical Gram-negative bacteria. The antibacterial experiments were performed according to the Antibacterial Standard of Japanese Association for the Functional Evaluation of Textiles. The fabrics modified with DNA/Ag nanoparticles showed a high enough inhibitory and killing efficiency against at a concentration of Ag ≥ 10 ppm.