Expression and significance of MMP3 in synovium of knee joint at different stage in osteoarthritis patients

• Published in: Asian Pacific journal of tropical medicine Vol. 7; no. 4; pp. 297 - 300
• Main Authors: Chen, Jun-Jie, Huang, Jie-Feng, Du, Wen-Xi, Tong, Pei-Jian
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 01.04.2014; Department of 0rthopedics, First Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou 310006, Zhejiang, China
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Analysis of Variance; Female; Humans; Immunohistochemical; Immunohistochemistry; Knee Joint - enzymology; Male; Matrix Metalloproteinase 3 - biosynthesis; Matrix Metalloproteinase 3 - chemistry; Matrix Metalloproteinase 3 - metabolism; Middle Aged; MMP-3; Osteoarthritis; Osteoarthritis - enzymology; Synovial Membrane - enzymology; Young Adult; Osteoarthritis; Immunohistochemical; MMP-3
• ISSN: 1995-7645; 2352-4146
• DOI: 10.1016/S1995-7645(14)60042-0

Objective:To investigate the expression and significance of MMP-3 in synovium of knee joint at different stage in osteoarthritis(OA)patients.Methods:Knee synovial tissue were collected in90 OA patients(the OA group).Patients in the OA group was divided into 3 subgroups:gradeⅠsubgroup(n=30),gradeⅡsubgroup(n=30),gradeⅢsubgroup(n=30).Thirty patients served as control group,lmmunohistochemical assay was used to detect the expression of MMP-3 protein in the knee synovial tissue.Results:MMP-3 protein was detected in all knee synovial tissue.The expression of MMP-3 protein in the OA group was significantly higher that in the normal synovium(P<0.05),and the MMP-3 protein was mainly located in the cytoplasm.There was significant difference in the expression of MMP-3 protein between the gradeⅢsubgroup and the gradeⅠ,gradeⅡsubgroups(all P<0.05).The expression of MMP-3 protein was positively related to the severity of OA(r=0.912,P<0.05).Conclusions:The expression of MMP-3 protein are closely related to pathogenic mechanism of OA.It may be an important indicator of early diagnosis and the activity of the disease of osteoarthritis.