DDB1 maintains intestinal homeostasis by preventing cell cycle arrest

Before the tissue collapse at day 4, the Ki67+ proliferating cells in the transient amplifying region of crypts were already decreased in the small intestine (Fig. 1D, S3A and S3B), while this change was delayed in the large intestine at day 6 (Fig. [...]the TUNEL assay revealed that cell death was...

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Bibliographic Details
Published inCell regeneration Vol. 11; no. 1; pp. 18 - 4
Main Authors Zhao, Lianzheng, Liao, Hongwei, Wang, Xiaodan, Chen, Ye-Guang
Format Journal Article
LanguageEnglish
Published Singapore Springer Nature Singapore 01.06.2022
Springer Nature B.V
SpringerOpen
Subjects
Animal models
Apoptosis
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell cycle
Cell death
Cell growth
Cell proliferation
Cyclin-dependent kinases
DDB1
Epithelium
G1 phase
Homeostasis
Intestine
Kinases
Large intestine
Letter
Life Sciences
Organoids
Proteins
Regenerative medicine
Small intestine
Stem Cells
DDB1
Homeostasis
Intestine
Cell cycle
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Summary:Before the tissue collapse at day 4, the Ki67+ proliferating cells in the transient amplifying region of crypts were already decreased in the small intestine (Fig. 1D, S3A and S3B), while this change was delayed in the large intestine at day 6 (Fig. [...]the TUNEL assay revealed that cell death was increased in the KO small intestine (Fig. [...]the decreased cell proliferation and increased death would contribute to the disruption of homeostasis. In summary, using the genetic mouse models and organoids, we demonstrate that DDB1 plays a critical role in the fast homeostatic renewal of the intestinal epithelium, which is achieved by reducing CDK inhibitor expression, preventing cell cycle arrest in the G1 phase and thus ensuring normal cell proliferation.
Bibliography:SourceType-Scholarly Journals-1
ObjectType-Correspondence-1
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ISSN:2045-9769
2045-9769
DOI:10.1186/s13619-022-00119-6