A Developmental Switch of AMPA Receptor Subunits in Neocortical Pyramidal Neurons

AMPA receptors mediate most of the fast excitatory neurotransmission in the brain, and those lacking the glutamate receptor 2 (GluR2) subunit are Ca(2+)-permeable and expressed in cortical structures primarily by inhibitory interneurons. Here we report that synaptic AMPA receptors of excitatory laye...

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Published inThe Journal of neuroscience Vol. 22; no. 8; pp. 3005 - 3015
Main Authors Kumar, Sanjay S, Bacci, Alberto, Kharazia, Viktor, Huguenard, John R
Format Journal Article
LanguageEnglish
Published United States Soc Neuroscience 15.04.2002
Society for Neuroscience
Subjects
Aging - metabolism
Animals
Biogenic Polyamines - metabolism
Biogenic Polyamines - pharmacology
Calcium - metabolism
Cell Membrane Permeability - physiology
Electric Stimulation
Excitatory Postsynaptic Potentials - drug effects
Excitatory Postsynaptic Potentials - physiology
In Vitro Techniques
Neocortex - cytology
Neocortex - growth & development
Neocortex - metabolism
Neuronal Plasticity - physiology
Patch-Clamp Techniques
Protein Subunits
Pyramidal Cells - drug effects
Pyramidal Cells - metabolism
Rats
Rats, Sprague-Dawley
Reaction Time - physiology
Receptors, AMPA - deficiency
Receptors, AMPA - metabolism
Synapses - metabolism
Synaptic Transmission - physiology
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Summary:AMPA receptors mediate most of the fast excitatory neurotransmission in the brain, and those lacking the glutamate receptor 2 (GluR2) subunit are Ca(2+)-permeable and expressed in cortical structures primarily by inhibitory interneurons. Here we report that synaptic AMPA receptors of excitatory layer 5 pyramidal neurons in the rat neocortex are deficient in GluR2 in early development, approximately before postnatal day 16, as evidenced by their inwardly rectifying current-voltage relationship, blockade of AMPA receptor-mediated EPSCs by external and internal polyamines, permeability to Ca(2+), and GluR2 immunoreactivity. Overall, these results indicate that neocortical pyramidal neurons undergo a developmental switch in the Ca(2+) permeability of their AMPA receptors through an alteration of their subunit composition. This has important implications for plasticity and neurotoxicity.
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ISSN:0270-6474
1529-2401
1529-2401
DOI:10.1523/jneurosci.22-08-03005.2002