Generation of a recombinant chickenized monoclonal antibody against the neuraminidase of H9N2 avian influenza virus

• Published in: AMB Express Vol. 10; no. 1; pp. 151 - 7
• Main Authors: Wang, Fei, Wang, Yajuan, Wan, Zhimin, Shao, Hongxia, Qian, Kun, Ye, Jianqiang, Qin, Aijian
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 20.08.2020; Springer Nature B.V; SpringerOpen
• Subjects: Antibodies; Antigens; Avian flu; Biomedical and Life Sciences; Biotechnology; Cell culture; Exo-a-sialidase; H9N2 AIV NA; Immunization; Immunization (passive); Influenza; Life Sciences; Microbial Genetics and Genomics; Microbiology; Monoclonal antibodies; NA inhibitory activity; Neutralizing ability; Original; Original Article; Poultry; Recombinant chickenized monoclonal antibody; Viruses; NA inhibitory activity; Recombinant chickenized monoclonal antibody; Neutralizing ability; H9N2 AIV NA
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-020-01086-4

We previously reported a monoclonal antibody (mAb), 1G8, against the neuraminidase (NA) of H9N2 avian influenza virus (AIV) with significant NA inhibitory activity. To generate a recombinant chickenized mAb (RCmAb) against the NA of H9N2 AIV for passive immunization in poultry, the gene of the fragment of antigen binding (Fab) of mAb 1G8 was cloned and fused with the fragment crystallizable (Fc) gene of chicken IgY. The RCmAb 1G8 was expressed in COS-1 cells and could be detected in cell culture supernatant. The results of NA inhibitory activity tests of the RCmAb 1G8 in an enzyme-linked lectin assay (ELLA) and a microneutralization (MN) assay showed that the RCmAb 1G8 maintained significant NA inhibitory activity and neutralizing ability. This is the first chickenized antibody against AIV, which would be a good candidate for passive immunization in poultry.