Characterization of Bacillus velezensis RDA1 as a Biological Control Agent against White Root Rot Disease Caused by Rosellinia necatrix

White root rot disease, caused by Rosellinia necatrix, poses a threat to several tree crops; hence, effective and sustainable strategies to control this disease remain warranted. This study identified an effective R. necatrix biocontrol agent by isolating 32 strains from soil samples collected from...

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Bibliographic Details
Published inPlants (Basel) Vol. 11; no. 19; p. 2486
Main Authors Sawant, Shailesh S, Janghoon Song, Ho-Jin Seo
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.09.2022
MDPI
Subjects
Acetic acid
Antifungal activity
Bacillus (Bacteria)
Bacillus velezensis
biocontrol
Biological control
Biosynthesis
Care and treatment
Control
Disease
Disease control
Environmental aspects
Fungi, Phytopathogenic
Fungicides
Gene sequencing
Genes
Identification and classification
Indoleacetic acid
Metabolites
Nucleotide sequence
Nucleotides
Orchards
Pests
Physiological aspects
Plant growth
Root rot
Root rots
Rosellinia necatrix
rRNA 16S
Solubilization
Strains (organisms)
Sustainable agriculture
Tree crops
Volatile compounds
white root rot
South Korea
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Summary:White root rot disease, caused by Rosellinia necatrix, poses a threat to several tree crops; hence, effective and sustainable strategies to control this disease remain warranted. This study identified an effective R. necatrix biocontrol agent by isolating 32 strains from soil samples collected from white root rot-infested organic pear orchards, among which RDA1 exhibited the most potent growth-inhibitory effects. Microbiological and 16S rRNA gene sequencing analyses revealed that the bacterial isolate belonged to the Bacillus genus and exhibited 100% nucleotide sequence similarity with Bacillus velezensis species in the GenBank. This strain showed strong antifungal activity against four Rosellinia necatrix strains and harbored genes essential for lipopeptide, polyketide, and tripeptide bacilysin biosynthesis. RDA1 produced volatile compounds that suppressed the development of phytopathogens and possessed plant growth-promoting traits, such as phosphate solubilization, and indole-3-acetic acid and siderophore production. B. velezensis RDA1 has a significant potential application in sustainable agriculture and can be used to suppress white root rot disease infections and to improve plant growth.
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ISSN:2223-7747
2223-7747
DOI:10.3390/plants11192486