Dual-color labeled anti-mucin 1 antibody for imaging of ovarian cancer: A preliminary animal study

To investigate the feasibility of the anti-mucin 1 (anti-MUC1/CD227) antibody in the fluorescent imaging of ovarian cancer, the CD227 antibody and a control IgG antibody were labeled with a near-infrared dye [Cy5.5-N-hydroxysuccinimide (NHS)] and a green dye (fluorescein-NHS). In vivo fluorescence i...

Full description

Saved in:
Bibliographic Details
Published inOncology letters Vol. 9; no. 3; pp. 1231 - 1235
Main Authors ZHANG, QIONG, WANG, FAN, WU, YAO-SEN, ZHANG, KE-KE, LIN, YAN, ZHU, XUE-QIONG, LV, JIE-QIANG, LU, XIAO-SHENG, ZHANG, XIAO-LEI, HU, YUE, HUANG, YIN-PING
Format Journal Article
LanguageEnglish
Published Greece D.A. Spandidos 01.03.2015
Spandidos Publications
Spandidos Publications UK Ltd
Subjects
Cancer
CD227 antibody
Cell culture
Chemokines
Dyes
FDA approval
Fluorescence
fluorescence imaging
Growth factors
Immunoglobulins
Medical imaging
Medical prognosis
mucin 1 antibody
near-infrared fluorescence
NMR
Nuclear magnetic resonance
Oncology
Oncology, Experimental
Ovarian cancer
Studies
Surgery
Tumors
Vitamin B
United States > US
near-infrared fluorescence
ovarian cancer
CD227 antibody
fluorescence imaging
mucin 1 antibody
Online AccessGet full text

Cover

More Information
Summary:To investigate the feasibility of the anti-mucin 1 (anti-MUC1/CD227) antibody in the fluorescent imaging of ovarian cancer, the CD227 antibody and a control IgG antibody were labeled with a near-infrared dye [Cy5.5-N-hydroxysuccinimide (NHS)] and a green dye (fluorescein-NHS). In vivo fluorescence images were obtained at 4, 12 and 36 h after injection of the probes into OVCAR3 tumor-bearing mice. The tumor to background ratios were calculated for both probes. Ex vivo fluorescence images were obtained following sacrifice at 36 h. After conjugation to Cy5.5 and fluorescein, the dual-color labeled CD227 probe (Ab-FL-Cy5.5) could be visualized by both green and near-infrared fluorescence. Uptake by the tumors was higher for the Ab-FL-Cy5.5 than for the IgG-Cy5.5 probe. All tumors could be visualized by in vivo imaging with an acceptable tumor to background ratio. Ex vivo studies demonstrated the advantages of using green fluorescence imaging to guide the resection of tumor tissues. These preliminary data indicate that the Ab-FL-Cy5.5 probe is promising for further tumor imaging applications and clinical translation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1792-1074
1792-1082
DOI:10.3892/ol.2014.2807