Genetic variation within coat color genes of MC1R and ASIP in Chinese brownish red Tibetan pigs
Melanocortin receptor 1 (MC1R) and agouti signaling protein (ASIP) are two major genes affecting coat color phenotypes in mammals, and inactivation mutations in the MC1R gene are responsible for red coat color in European pig breeds. Conversely, the gain‐of‐function ASIP mutations block MC1R signali...
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Published in | Animal science journal Vol. 81; no. 6; pp. 630 - 634 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Melbourne, Australia
Blackwell Publishing Asia
01.12.2010
Blackwell Publishing Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Melanocortin receptor 1 (MC1R) and agouti signaling protein (ASIP) are two major genes affecting coat color phenotypes in mammals, and inactivation mutations in the MC1R gene are responsible for red coat color in European pig breeds. Conversely, the gain‐of‐function ASIP mutations block MC1R signaling and lead to the production of red pheomelanin. Chinese Tibetan pigs have three types of coat color phenotypes, including brownish red, solid black and black with patches of brownish red and white. Herein, we investigated variations of the MC1R and ASIP genes in Tibetan pigs. The results showed that the brownish red Tibet pig had the dominant black MC1R allele (ED¹). No loss‐of‐function mutation in MC1R responsible for red coat color in European breeds was observed in this breed. No causal mutation for the red coat color phenotype was found in the coding sequence of the ASIP gene. A novel missense mutation c.157G > A was firstly identified in exon 2 of ASIP, which was further genotyped in 285 pigs from five Chinese breeds and three Western breeds having different coat color phenotypes. Nearly all pigs were GG homozygotes. In conclusion, no functional variant responsible for brownish red coloration was found in the coding region of MC1R and ASIP in Tibetan pigs. |
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Bibliography: | http://dx.doi.org/10.1111/j.1740-0929.2010.00789.x ark:/67375/WNG-N8S3SWZX-M ArticleID:ASJ789 istex:CBBE0E8927E57C501F9835B96E0D25F480EB552F These authors contributed equally to this work and should be considered co‐first authors. The coding sequences of MC1R and ASIP in Chinese brownish red Tibetan pigs have been submitted to GenBank with accession numbers GQ373178 and GQ373180. ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 1344-3941 1740-0929 |
DOI: | 10.1111/j.1740-0929.2010.00789.x |