Draft genome sequence of the New Jersey aster yellows strain of 'Candidatus Phytoplasma asteris'

The NJAY (New Jersey aster yellows) strain of 'Candidatus Phytoplasma asteris' is a significant plant pathogen responsible for causing severe lettuce yellows in the U.S. state of New Jersey. A draft genome sequence was prepared for this organism. A total of 177,847 reads were assembled int...

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Published inPloS one Vol. 13; no. 2; p. e0192379
Main Authors Sparks, Michael E, Bottner-Parker, Kristi D, Gundersen-Rindal, Dawn E, Lee, Ing-Ming
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 06.02.2018
Public Library of Science (PLoS)
Subjects
Bioinformatics
Biology and Life Sciences
Candidatus Phytoplasma asteris
Flowers & plants
Genes
Genetic aspects
Genomes
Genomics
Homology
Laboratories
Nucleotide sequence
Pathogenicity
Pathogens
Phylogenetics
Phylogeny
Phytoplasma
Plant bacterial diseases
Plant diseases
Plant pathology
Plasmids
Proteins
Research and Analysis Methods
Virulence
Virulence factors
Witches' broom
Yellows
United States
United States > US
Maryland
New Jersey
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Summary:The NJAY (New Jersey aster yellows) strain of 'Candidatus Phytoplasma asteris' is a significant plant pathogen responsible for causing severe lettuce yellows in the U.S. state of New Jersey. A draft genome sequence was prepared for this organism. A total of 177,847 reads were assembled into 75 contigs > 518 bp with a total base value of 652,092 and an overall [G+C] content of 27.1%. A total of 733 protein coding genes were identified. This Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession MAPF00000000. This draft genome was used for genome- and gene-based comparative phylogenetic analyses with other phytoplasmas, including the closely related 'Ca. Phytoplasma asteris' strain, aster yellows witches'- broom (AY-WB). NJAY and AY-WB exhibit approximately 0.5% dissimilarity at the nucleotide level among their shared genomic segments. Evidence indicated that NJAY harbors four plasmids homologous to those known to encode pathogenicity determinants in AY-WB, as well as a chromosome-encoded mobile unit. Apparent NJAY orthologs to the important AY-WB virulence factors, SAP11 and SAP54, were identified. A number of secreted proteins, both membrane-bound and soluble, were encoded, with many bearing similarity to known AY-WB effector molecules and others representing possible secreted proteins that may be novel to the NJAY lineage.
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Competing Interests: The views expressed in this article are those of the authors and do not necessarily reflect the official policy or position of the US government. All authors are employees of the U.S. Government. This work was prepared as part of their official duties. Title 17 U.S.C. §105 provides that ‘Copyright protection under this title is not available for any work of the United States Government.’ Title 17 U.S.C. §101 defines a US Government work as a work prepared by a military Service Member or employee of the US Government as part of that person’s official duties.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0192379