Age- and sex-dependence of dopamine release and capacity for recovery identified in the dorsal striatum of C57/Bl6J mice

• Published in: PloS one Vol. 9; no. 6; p. e99592
• Main Authors: Arvidsson, Emma, Viereckel, Thomas, Mikulovic, Sanja, Wallén-Mackenzie, Åsa
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12.06.2014; Public Library of Science (PLoS)
• Subjects: Addictions; Age; Age Factors; Aging - metabolism; Aging - psychology; Amplitudes; Analysis; Animals; Basal ganglia; Biology and Life Sciences; Brain research; Caudate-putamen; Chlorides; Corpus Striatum - cytology; Corpus Striatum - metabolism; Corpus Striatum - physiology; Dopamine; Dopamine - metabolism; Dopamine receptors; Dopaminergic Neurons - metabolism; Dopaminergic Neurons - physiology; Female; Females; Ganglia; Gender differences; Gilles de la Tourette syndrome; Kinetics; Male; Males; Mathematical models; Mental disorders; Mice; Mice, Inbred C57BL; Movement disorders; Neostriatum; Neurodegenerative diseases; Neurological diseases; Neurosciences; Obsessive compulsive disorder; Parameter identification; Parkinson's disease; Phenols (Class of compounds); Potassium chloride; Recovery; Recovery (Medical); Recovery of Function - physiology; Rodents; Sex; Sex Factors; Stimulation; Substantia nigra; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0099592

The dorsal striatum is the main input structure of the basal ganglia and the major target area of dopaminergic projections originating in the substantia nigra pars compacta. Heavily involved in the regulation of voluntary movement and habit formation, this structure is of strong importance in Parkinson's disease, obsessive-compulsive disorder, Tourette's syndrome and addiction. The C57/Bl6J mouse strain, the most commonly used strain in preclinical research today, is frequently used as a model organism for analysis of dopaminergic parameters implicated in human pathophysiology. Several components of the dopamine system have been shown to vary with age and sex, however knowledge of the contribution of these factors for dopamine release kinetics in the C57/Bl6J mouse strain is lacking. In the present study, we used an intracranial KCl-stimulation challenge paradigm to provoke release from dopaminergic terminals in the dorsal striatum of anaesthetized C57/Bl6J mice. By high-speed in vivo chronoamperometric recordings, we analyzed DA release parameters in male and female mice of two different ages. Our experiments demonstrate elevated DA amplitudes in adult compared to young mice of both sexes and higher DA amplitudes in females compared to males at both ages. Adult mice exhibited higher recovery capabilities after repeated stimulation than did young mice and also showed a lower variability in the kinetic parameters trise and t80 between stimulations. These results identified age- and sex- dimorphisms in DA release parameters and point to the importance of taking these dimorphisms into account when utilizing the C57/Bl6J mouse strain as model for neurological and neuropsychiatric disorders.