p53 dependent apoptotic cell death induces embryonic malformation in Carassius auratus under chronic hypoxia

• Published in: PloS one Vol. 9; no. 7; p. e102650
• Main Authors: Sawant, Paramita Banerjee, Bera, Aritra, Dasgupta, Subrata, Sawant, Bhawesh T, Chadha, Narinder K, Pal, Asim K
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.07.2014; Public Library of Science (PLoS)
• Subjects: Animals; Apoptosis; Apoptosis - physiology; Aquatic animals; Aquatic fauna; Bcl-2 protein; Biochemistry; Biology and Life Sciences; Cancer; Carassius auratus; Caspase; Caspase 3 - metabolism; Caspase-3; Cell cycle; Cell death; Cell Proliferation; Chronic Disease; Comparative analysis; Congenital Abnormalities; Deoxyribonucleic acid; Developmental biology; DNA; DNA damage; Embryogenesis; Embryonic development; Embryonic growth stage; Embryos; Fauna; Fertilization; Fish; Fisheries; Fishes; Gene expression; Goldfish - embryology; Hypoxia; Hypoxia - pathology; Males; Medical research; Mortality; p53 Protein; Proto-Oncogene Proteins c-bcl-2 - metabolism; Somites; Tumor proteins; Tumor suppressor genes; Tumor Suppressor Protein p53 - physiology; Zebrafish; Mumbai India; India
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0102650

Hypoxia is a global phenomenon affecting recruitment as well as the embryonic development of aquatic fauna. The present study depicts hypoxia induced disruption of the intrinsic pathway of programmed cell death (PCD), leading to embryonic malformation in the goldfish, Carrasius auratus. Constant hypoxia induced the early expression of pro-apoptotic/tumor suppressor p53 and concomitant expression of the cell death molecule, caspase-3, leading to high level of DNA damage and cell death in hypoxic embryos, as compared to normoxic ones. As a result, the former showed delayed 4 and 64 celled stages and a delay in appearance of epiboly stage. Expression of p53 efficiently switched off expression of the anti-apoptotic Bcl-2 during the initial 12 hours post fertilization (hpf) and caused embryonic cell death. However, after 12 hours, simultaneous downregulation of p53 and Caspase-3 and exponential increase of Bcl-2, caused uncontrolled cell proliferation and prevented essential programmed cell death (PCD), ultimately resulting in significant (p<0.05) embryonic malformation up to 144 hpf. Evidences suggest that uncontrolled cell proliferation after 12 hpf may have been due to downregulation of p53 abundance, which in turn has an influence on upregulation of anti-apoptotic Bcl-2. Therefore, we have been able to show for the first time and propose that hypoxia induced downregulation of p53 beyond 12 hpf, disrupts PCD and leads to failure in normal differentiation, causing malformation in gold fish embryos.