侵染广东黄秋葵的木尔坦棉花曲叶病毒及伴随卫星DNA的分子特征

从广东省表现黄脉曲叶的黄秋葵病株上分离到病毒分离物Okra06，PCR检测结果显示，该病毒属双生病毒科Geminiviridae菜豆金色花叶病毒属Begomovirus．基因克隆及序列分析结果表明，其基因组仅含A组分（DNA—A），全长为2737m，推导编码6个开放阅读框（Open reading frame，ORF）．该组分与木尔坦棉花曲叶病毒（Cotton leaf curl Multan virus, CLCuMV）分离物G6的核苷酸序列相似性最高，为99．7％；二者编码的6个ORF相似性分别为100％、100％、99．6％、99．8％、100％和99．7％．该病毒还伴随有卫星分子B（D...

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Bibliographic Details
Published in华南农业大学学报 Vol. 33; no. 1; pp. 33 - 39
Main Author 董迪 朱艳华 何自福 柴兆祥 佘小漫 罗方芳
Format Journal Article
LanguageChinese
Published 甘肃农业大学草业学院,甘肃兰州730070%广东省农业科学院植物保护研究所,广东广州,510640%甘肃农业大学草业学院,甘肃兰州,730070 2012
广东省农业科学院植物保护研究所,广东广州510640
Subjects
DNA—A
β
卫星DNA
木尔坦棉花曲叶病毒
黄秋葵黄脉曲叶病
木尔坦棉花曲叶病毒
DNA-A
黄秋葵黄脉曲叶病
卫星DNA β
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ISSN1001-411X
DOI10.3969/j.issn.1001-411X.2012.01.007

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Summary:从广东省表现黄脉曲叶的黄秋葵病株上分离到病毒分离物Okra06，PCR检测结果显示，该病毒属双生病毒科Geminiviridae菜豆金色花叶病毒属Begomovirus．基因克隆及序列分析结果表明，其基因组仅含A组分（DNA—A），全长为2737m，推导编码6个开放阅读框（Open reading frame，ORF）．该组分与木尔坦棉花曲叶病毒（Cotton leaf curl Multan virus, CLCuMV）分离物G6的核苷酸序列相似性最高，为99．7％；二者编码的6个ORF相似性分别为100％、100％、99．6％、99．8％、100％和99．7％．该病毒还伴随有卫星分子B（DNA13），全长为1346nt，推导其互补链上编码1个ORF（C1）．该分子与伴随CLCuMV分离物G6DNAβ的核苷酸序列相似性也最高（99．5％）．DNAp系统进化分析显示，Okra06DNAβ与CLCuMV-[G6]DNAβ、CLCuMV-[Gx08]DNAβ亲缘关系最近，三者形成一个独立分支；进一步与其他CLCuMVDNAβ和CLCuVDNAβ聚类在一起．这些结果证明，侵染广东黄秋葵的病毒分离物Okra06属于CLCuMV，且该分离物与引起广东朱槿曲叶病的CLCuMV—G6应同属木尔坦棉花曲叶病毒朱槿株系．
Bibliography:The virus isolate Okra06 was obtained from the diseased okra plants exhibiting yellow vein and leaf curl symptoms in Guangzhou, Guangdong Province. The results of PCR detection indicated that the virus belonged to the genus Begomovirus of the family Geminiviridae. The genome of the virus was cloned and sequenced. The results showed that it had only A component（DNA-A）. The complete nueleotide sequence of DNA-A was determined to be 2 737 nucleotides, encoding six potential ORFs. The comparison showed that Okra06 DNA-A had the highest nucleotide sequence identity （99.7%） with CLCuMV- [ C,6]. Six ORFs shared 100% , 100% , 99.6% , 99.8% , 100% and 99.7% nucleotide sequence identities with CLCuMV - [ C,6 ] , respectively. The Okra06 was also associated with satellite DNA 13 molecular. The full-length sequence of DNA 13 was determined to be 1 346 nucleotides, encoding one potential ORF （C1）. The comparison further showed that Okra06 DNAβ had the highest nucleotide sequence identity （99.5 % ）with C,6 DNA β. Phylogene
ISSN:1001-411X
DOI:10.3969/j.issn.1001-411X.2012.01.007