一株高活性黑木耳酿酒酵母的鉴定及其相关生物学特性分析

为研究黑木耳发酵酿酒试验引进多株酵母菌，对一株利用黑木耳酿酒活性较高的酵母进行生理生化特性研究，为黑木耳发酵酿酒产业储备菌种资源。将待测酵母标记为J800，结合形态特征和26S rDNA D1/D2区序列分析，将该菌株鉴定为酿酒酵母菌（Saccharomyces cerevisiae sp.），此菌适宜在弱酸性环境中生长繁殖，最适生长条件：pH 4.6、32℃、阳离子浓度0.7%~1.0%；当酒精含量达到15%以上，酵母生长受到较强抑制；发酵力0.62~0.70，培养基中蔗糖含量为20%时达到最大。...

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Published in东北农业大学学报 Vol. 45; no. 6; pp. 45 - 48
Main Author 臧海莲 邢振楠 范冬茹 王丹丽 熊明华
Format Journal Article
LanguageChinese
Published 东北农业大学资源与环境学院，哈尔滨 150030%伊春林业科学院,黑龙江 伊春,153000%淮北师范大学生命科学学院,安徽 淮北,235000 2014
伊春林业科学院，黑龙江 伊春 153000
Subjects
序列分析
生物学特性
酿酒酵母
生物学特性
序列分析
sequence analysis
biological characteristics
酿酒酵母
Saccharomyces cerevisiae
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Summary:为研究黑木耳发酵酿酒试验引进多株酵母菌，对一株利用黑木耳酿酒活性较高的酵母进行生理生化特性研究，为黑木耳发酵酿酒产业储备菌种资源。将待测酵母标记为J800，结合形态特征和26S rDNA D1/D2区序列分析，将该菌株鉴定为酿酒酵母菌（Saccharomyces cerevisiae sp.），此菌适宜在弱酸性环境中生长繁殖，最适生长条件：pH 4.6、32℃、阳离子浓度0.7%~1.0%；当酒精含量达到15%以上，酵母生长受到较强抑制；发酵力0.62~0.70，培养基中蔗糖含量为20%时达到最大。
Bibliography:23-1391/S
For researching alcohol fermentation using black agaric as ingredients, several yeasts were purchased. One yeast, named as J800, showing higher fermentation capability was subject to further study. Combining morphologic and physiobiochemical characteristics with 26S rDNA sequence analysis, strain J800 was identified as Saccharomyces cerevisiae sp.. The optimal growth condition for strain J800 was pH 4.6, 32℃, 0.7%-1.0%cation concentration. In addition, when alcohol concentration reached 15%or more in fermentation broth, the growth of strain J800 was strongly inhibited. The strain J800 reached the maximum fermentation force（0.62-0.70） at the sucrose concentration 20% in the medium.
ZANG Hailian, XlNG Zhennan, FAN Dongru, WANG Danli, XlONG Minghua（1. Yichun Academy of Forestry Sciences, Yichun Heilongjiang 153000, China; 2. School of Resources and Environmental Sciences, Northeast Agricultural University, Harbin 150030, China; 3. School of Life Sciences, Huaibei Normal Universitv. Huaibei Anhui 235000.
ISSN:1005-9369