Direct sequencing of hepatitis A virus strains isolated during an epidemic in France

• Published in: Applied and Environmental Microbiology Vol. 61; no. 11; pp. 3977 - 3980
• Main Authors: Apaire-Marchais, V, Robertson, B H, Aubineau-Ferre, V, Le Roux, M G
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.11.1995
• Subjects: Adolescent; Adult; alimentos; Amino Acid Sequence; Base Sequence; Biological and medical sciences; biological contamination; cardium; chemical composition; Child; composicion quimica; composition chimique; contaminacion; contaminacion biologica; contamination; contamination biologique; coquillages et crustaces; Disease Outbreaks; DNA, Viral - genetics; enfermedades transm por alimentos; enterovirus; Epidemics; epidemiologia; epidemiologie; Epidemiology; faeces; feces; Feces - virology; Female; foodborne diseases; foods; france; France - epidemiology; francia; Fundamental and applied biological sciences. Psychology; genero humano; Genetic Variation; genomas; genome; genomes; Genotype; genre humain; heces; hepatite; Hepatitis; Hepatitis A - epidemiology; Hepatitis A - virology; hepatitis A virus; Hepatovirus - classification; Hepatovirus - genetics; Hepatovirus - isolation & purification; Humans; maladie transmissible par aliment; Male; mankind; mariscos; Microbiology; Molecular biology; Molecular Sequence Data; nucleotide sequence; Polymerase Chain Reaction; produit alimentaire; proteinas; proteine; proteins; secuencia nucleica; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; sequence nucleique; shellfish; Viral Structural Proteins - genetics; Virology; virose; viroses; virosis; virus; viruses; France; Typing; Hepatitis A virus; Shellfish; Nucleotide sequence; Picornaviridae; Method; Epidemiology; Virus; Polymerase chain reaction; Environment; Genome; Clinical isolate; Comparative study; Aminoacid sequence
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.61.11.3977-3980.1995

Direct sequencing of PCR products was used to study the VP1 region of the hepatitis A virus (HAY) genome (position 2199 to 2356) of nine strains isolated from human stools collected during a hepatitis A epidemic (western France, 1992), three strains from environmental samples (1990, 1991, and 1992), and two HAV cell culture isolates (the French strain CF53/Lyon and strain CLF). These viruses differed from CF53/Lyon (genotype I) by between 1 and 10.3%, and results indicated the existence of two groups of strains belonging to two different subgenotypes (IA and IB). With this sequencing technique it was possible to monitor the epidemiology of HAV and study its relations.