Immunochemical Measurement of Placental Tissue Protein 4 in Serum

• Published in: Chemical & pharmaceutical bulletin Vol. 34; no. 5; pp. 2191 - 2195
• Main Authors: FUKAZAWA, ICHIO, TAKAMIZAWA, HIROYOSHI, SHIROTAKE, SYOICHI, OHTA, YORIKO, BOHN, HANS, INABA, NORIYUKI
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 1986; Maruzen; Japan Science and Technology Agency
• Subjects: Analytical, structural and metabolic biochemistry; Annexin A5; Biological and medical sciences; endometrial cancer; Female; Fundamental and applied biological sciences. Psychology; Holoproteins; Humans; Immunoenzyme Techniques; Other proteins; Pregnancy; Pregnancy Proteins - analysis; Pregnancy Proteins - blood; Proteins; Radioimmunoassay; Human; Serum; Tumor; Placenta; Enzyme immunoassay; Blood
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.34.2191

Using the avidin-biotin binding system, an enzyme immunoassay procedure was developed to measure the placental tissue protein 4 (PP4) in serum. The standard curve covered the range from 0.5 to 100 ng/ml of PP4. The intra- and inter-assay coefficients of variation were less than 5 and 10%, respectively. Recoveries of PP4 added to serum ranged between about 99 and 105%. The result obtained by this assay method correlated well with those obtained by the radioimmunoassay method. The PP4 serum leve was under 10 ng/ml in non-pathological individuals. In pregnancies during 5-40 weeks, the level ranged from 0.9 to 15.0 ng/ml. In ovarian malignancies, the PP4 concentration in serum irrespectively scattered from 0.9 to 21 ng/ml. Significantly high PP4 levels were found in uterine cervical cancer in the early stage and also in endometrial cancer.