Serological study using glycoarray for detecting antibodies to glycolipids and glycolipid complexes in immune-mediated neuropathies

• Published in: Journal of neuroimmunology Vol. 301; pp. 35 - 40
• Main Authors: Morikawa, Miyuki, Kuwahara, Motoi, Ueno, Rino, Samukawa, Makoto, Hamada, Yukihiro, Kusunoki, Susumu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.12.2016
• Subjects: Adult; Aged; Aged, 80 and over; Allergy and Immunology; Antibodies; Autoantibodies - blood; Enzyme-Linked Immunosorbent Assay; Female; Gangliosides - immunology; Glycoarray; Glycolipid complex; Glycolipids - immunology; Guillain-Barre Syndrome - blood; Guillain-Barré syndrome; Humans; Immunoglobulin G - blood; Japan; Male; Middle Aged; Multifocal motor neuropathy; Neurology; Polyneuropathies - blood; Polyradiculoneuropathy, Chronic Inflammatory Demyelinating - blood; Statistics, Nonparametric; Sulfoglycosphingolipids - immunology; Young Adult; Antibodies; Glycolipid complex; Multifocal motor neuropathy; Glycoarray; Guillain-Barré syndrome
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/j.jneuroim.2016.10.010

Abstract We performed a serological investigation using glycoarray in Guillain-Barré syndrome (GBS), chronic inflammatory demyelinating polyradiculoneuropathy (CIDP), and multifocal motor neuropathy (MMN). Antibodies to 10 glycolipids and 45 glycolipid complexes were tested. Anti-GM1/sulfatide and anti-GA1/sulfatide IgG antibodies were common in GBS (20.0% and 19.0%, respectively). Anti-GQ1b/sulfatide IgG antibody was detected in 14.0% of GBS patients. IgG antibodies to antigens containing GQ1b were significantly correlated with ophthalmoplegia in GBS (p < 0.01). IgM antibodies to antigens containing GM1 or GalNAc-GD1a were in 50% and 37.5% of MMN patients, respectively. Glycoarray is efficient for detecting antibodies against numerous glycolipid complexes in immune-mediated neuropathies.