A reference genetic map of Muscadinia rotundifolia and identification of Ren5, a new major locus for resistance to grapevine powdery mildew

• Published in: Theoretical and applied genetics Vol. 125; no. 8; pp. 1663 - 1675
• Main Authors: Blanc, Sophie, Wiedemann-Merdinoglu, Sabine, Dumas, Vincent, Mestre, Pere, Merdinoglu, Didier
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.12.2012; Springer; Springer Nature B.V; Springer Verlag
• Subjects: 19th century; Agriculture; Ascomycetes; Ascomycota; Ascomycota - physiology; Ascomycota - ultrastructure; Biochemistry; Biomedical and Life Sciences; Biotechnology; chromosome mapping; Chromosome Mapping - methods; chromosome number; Chromosomes; Cultivars; Cultural heritage; Development and progression; Disease Resistance; Disease Resistance - genetics; Diseases and pests; Erysiphe necator; Fungicides; Genetic aspects; Genetic Linkage; Genetic Loci; Genetic Loci - genetics; genetics; genome; Genome, Plant; Genome, Plant - genetics; Genomes; Genotype; Grapes; growth & development; immunology; Life Sciences; linkage groups; loci; methods; microbiology; microsatellite repeats; Mildew; Mycelium; Mycelium - growth & development; Mycelium - ultrastructure; Oomycetes; Original Paper; pathogens; Pesticides; phenotypic variation; Physiological aspects; physiology; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant immunology; Plasmopara viticola; powdery mildew; progeny; quantitative trait loci; Quantitative Trait Loci - genetics; Reference Standards; Synteny; Synteny - genetics; ultrastructure; Uncinula necator; Vitaceae; Vitis; Vitis - genetics; Vitis - immunology; Vitis - microbiology; Vitis - ultrastructure; Vitis vinifera; France; Europe; Quantitative Trait Locus; Simple Sequence Repeat Marker; Powdery Mildew; Downy Mildew; Quantitative Trait Locus Analysis; Vitis vinifera; Genetic; Muscadinia rotundifolia; Ren5; Rresistance to grapevine powdery mildew
• ISSN: 0040-5752; 1432-2242; 1432-2242
• DOI: 10.1007/s00122-012-1942-3

Muscadinia rotundifolia , a species closely related to cultivated grapevine Vitis vinifera , is a major source of resistance to grapevine downy and powdery mildew, two major threats to cultivated traditional cultivars of V. vinifera respectively caused by the oomycete Plasmopara viticola and the ascomycete Erisyphe necator . The aim of the present work was to develop a reference genetic linkage map based on simple sequence repeat (SSR) markers for M. rotundifolia . This map was created using S1 M. rotundifolia cv. Regale progeny, and covers 948 cM on 20 linkage groups, which corresponds to the expected chromosome number for muscadine. The comparison of the genetic maps of V. vinifera and M. rotundifolia revealed a high macrosynteny between the genomes of both species. The S1 progeny was used to assess the general level of resistance of M. rotundifolia to P. viticola and E. necator , by scoring different parameters of pathogen development. A quantitative trait locus (QTL) analysis allowed us to highlight a major QTL on linkage group 14 controlling resistance to powdery mildew, which explained up to 58 % of the total phenotypic variance. This QTL was named ‘Resistance to Erysiphe Necator 5 ’ ( Ren5 ). A microscopic evaluation E. necator mycelium development on resistant and susceptible genotypes of the S1 progeny showed that Ren5 exerts its action after the formation of the first appressorium, and acts by delaying, and then stopping, mycelium development.