Enhanced skin wound healing by a sustained release of growth factors contained in platelet-rich plasma

• Published in: Experimental & molecular medicine Vol. 43; no. 11; pp. 622 - 629
• Main Authors: Yang, Hee Seok, Shin, Jaehoon, Bhang, Suk Ho, Shin, Jung-Youn, Park, Jooyeon, Im, Gun-Il, Kim, Chang-Sung, Kim, Byung-Soo
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 2011; Springer Nature B.V; Korean Society for Biochemistry and Molecular Biology; 생화학분자생물학회
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Blotting, Western; Cell Proliferation; Dermis - cytology; Dermis - metabolism; Female; Fibrin - metabolism; Fibroblast Growth Factor 2 - genetics; Fibroblast Growth Factor 2 - metabolism; Heparin - metabolism; Immunoenzyme Techniques; Intercellular Signaling Peptides and Proteins - secretion; Medical Biochemistry; Mice; Mice, Inbred BALB C; Molecular Medicine; Original; Platelet-Rich Plasma - metabolism; Proto-Oncogene Proteins c-sis - genetics; Proto-Oncogene Proteins c-sis - metabolism; Real-Time Polymerase Chain Reaction; Regeneration; RNA, Messenger - genetics; Skin - cytology; Skin - metabolism; Stem Cells; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; Wound Healing - physiology; 생화학; heparin; wound healing; fibroblast growth factor 2; platelet-derived growth factor; endothelial growth factors; fibrin; platelet-rich plasma; angiogenesis inducing agents; neovascularization, physiologic
• ISSN: 1226-3613; 2092-6413
• DOI: 10.3858/emm.2011.43.11.070

Platelet-rich plasma (PRP) contains growth factors that promote tissue regeneration. Previously, we showed that heparin-conjugated fibrin (HCF) exerts the sustained release of growth factors with affinity for heparin. Here, we hypothesize that treatment of skin wound with a mixture of PRP and HCF exerts sustained release of several growth factors contained in PRP and promotes skin wound healing. The release of fibroblast growth factor 2, platelet-derived growth factor-BB, and vascular endothelial growth factor contained in PRP from HCF was sustained for a longer period than those from PRP, calcium-activated PRP (C-PRP), or a mixture of fibrin and PRP (F-PRP). Treatment of full-thickness skin wounds in mice with HCF-PRP resulted in much faster wound closure as well as dermal and epidermal regeneration at day 12 compared to treatment with either C-PRP or F-PRP. Enhanced skin regeneration observed in HCF-PRP group may have been at least partially due to enhanced angiogenesis in the wound beds. Therefore, this method could be useful for skin wound treatment.