Single-cell transcriptomic reveals molecular diversity and developmental heterogeneity of human stem cell-derived oligodendrocyte lineage cells

• Published in: Nature communications Vol. 12; no. 1; p. 652
• Main Authors: Chamling, Xitiz, Kallman, Alyssa, Fang, Weixiang, Berlinicke, Cynthia A., Mertz, Joseph L., Devkota, Prajwal, Pantoja, Itzy E. Morales, Smith, Matthew D., Ji, Zhicheng, Chang, Calvin, Kaushik, Aniruddha, Chen, Liben, Whartenby, Katharine A., Calabresi, Peter A., Mao, Hai-Quan, Ji, Hongkai, Wang, Tza-Huei, Zack, Donald J.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 28.01.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 101/62; 13/100; 13/106; 13/31; 13/51; 38/39; 38/77; 38/91; 42/41; 45/41; 45/88; 631/136/368/2430; 631/1647/514/1949; 631/378/2596/1308; 631/378/2596/1705; 631/532/2064; 96/35; Astrocytes; Astrocytes - cytology; Astrocytes - metabolism; Biosynthesis; Cell differentiation; Cell Differentiation - genetics; Cell Line; Cell Lineage - genetics; Cholesterol; Cholesterol - biosynthesis; Cytokines; Demyelinating diseases; Demyelination; Embryonic Stem Cells - cytology; Embryonic Stem Cells - metabolism; Gene Regulatory Networks - genetics; Gene sequencing; Genes; Genetic Heterogeneity; Genetic Variation; Genomes; Glial stem cells; Heterogeneity; Humanities and Social Sciences; Humans; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; multidisciplinary; Multiple sclerosis; Myelination; Oligodendrocyte Precursor Cells - cytology; Oligodendrocyte Precursor Cells - metabolism; Oligodendrocytes; Pluripotency; Populations; Progenitor cells; Purification; Receptor, Platelet-Derived Growth Factor alpha - genetics; Receptor, Platelet-Derived Growth Factor alpha - metabolism; Ribonucleic acid; RNA; Science; Science (multidisciplinary); Signal Transduction - genetics; Single-Cell Analysis - methods; Stem cells; TOR protein; TOR Serine-Threonine Kinases - genetics; TOR Serine-Threonine Kinases - metabolism; Trajectory analysis; Transcription; Transcriptome - genetics; Transcriptomics
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-20892-3

Injury and loss of oligodendrocytes can cause demyelinating diseases such as multiple sclerosis. To improve our understanding of human oligodendrocyte development, which could facilitate development of remyelination-based treatment strategies, here we describe time-course single-cell-transcriptomic analysis of developing human stem cell-derived oligodendrocyte-lineage-cells (hOLLCs). The study includes hOLLCs derived from both genome engineered embryonic stem cell (ESC) reporter cells containing an Identification-and-Purification tag driven by the endogenous PDGFRα promoter and from unmodified induced pluripotent (iPS) cells. Our analysis uncovers substantial transcriptional heterogeneity of PDGFRα-lineage hOLLCs. We discover sub-populations of human oligodendrocyte progenitor cells (hOPCs) including a potential cytokine-responsive hOPC subset, and identify candidate regulatory genes/networks that define the identity of these sub-populations. Pseudotime trajectory analysis defines developmental pathways of oligodendrocytes vs astrocytes from PDGFRα-expressing hOPCs and predicts differentially expressed genes between the two lineages. In addition, pathway enrichment analysis followed by pharmacological intervention of these pathways confirm that mTOR and cholesterol biosynthesis signaling pathways are involved in maturation of oligodendrocytes from hOPCs. Brain myelinating oligodendrocytes are rare and difficult to isolate, which has limited data on their development. Here the authors develop a reporter for scalable purification of human pluripotent stem cell derived oligodendrocyte lineage cells, and use this to map differentiation using single cell RNA-sequencing,