A four-microRNA classifier as a novel prognostic marker for tumor recurrence in stage II colon cancer

• Published in: Scientific reports Vol. 8; no. 1; pp. 6157 - 9
• Main Authors: Jacob, Havjin, Stanisavljevic, Luka, Storli, Kristian Eeg, Hestetun, Kjersti E., Dahl, Olav, Myklebust, Mette P.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.04.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 631/337/384/331; 631/61/514/2254; 631/67/1504/1885/1393; Aged; Aged, 80 and over; Biomarkers; Biomarkers, Tumor; Chemotherapy; Colon cancer; Colonic Neoplasms - diagnosis; Colonic Neoplasms - genetics; Colonic Neoplasms - mortality; Colorectal cancer; Female; Humanities and Social Sciences; Humans; Kaplan-Meier Estimate; Male; MicroRNAs; MicroRNAs - genetics; Middle Aged; miRNA; multidisciplinary; Neoplasm Recurrence, Local; Neoplasm Staging; Nomograms; Prognosis; Proportional Hazards Models; Risk factors; Risk groups; ROC Curve; Science; Science (multidisciplinary); Surgery
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-018-24519-4

About 20 percent of TNM-stage II colon cancer patients who are treated by surgical resection develop recurrence, and adjuvant chemotherapy in this group is still debated among researchers and clinicians. Currently, adverse histopathological and clinical factors are used to select patients for adjuvant chemotherapy following surgery. However, additional biomarkers to classify patients at risk of recurrence are needed. We have conducted a study using fresh frozen tumor tissue from 54 TNM-stage II colon cancer patients and performed microRNA profiling using next-generation sequencing. For the selection of the prognostic microRNAs, a LASSO Cox Regression model was employed. For the validation, we used the publically available TCGA-COAD cohort (n = 122). A prognostic panel of four micorRNAs (hsa-miR-5010-3p, hsa-miR-5100, hsa-miR-656-3p and hsa-miR-671-3p) was identified in the study cohort and validated in the TCGA-COAD cohort. The four-microRNA classifier successfully identified high-risk patients in the study cohort (P < 0.001) and the validation cohort (P = 0.005). Additionally, a number of established risk factors and the four-miRNA classifier were used to construct a nomogram to evaluate risk of recurrence. We identified a four-microRNA classifier in patients with TNM-stage II colon cancer that can be used to discriminate between patients at low- and high risk of recurrence.