Evidence for SARS-CoV-2 related coronaviruses circulating in bats and pangolins in Southeast Asia

• Published in: Nature communications Vol. 12; no. 1; p. 972
• Main Authors: Wacharapluesadee, Supaporn, Tan, Chee Wah, Maneeorn, Patarapol, Duengkae, Prateep, Zhu, Feng, Joyjinda, Yutthana, Kaewpom, Thongchai, Chia, Wan Ni, Ampoot, Weenassarin, Lim, Beng Lee, Worachotsueptrakun, Kanthita, Chen, Vivian Chih-Wei, Sirichan, Nutthinee, Ruchisrisarod, Chanida, Rodpan, Apaporn, Noradechanon, Kirana, Phaichana, Thanawadee, Jantarat, Niran, Thongnumchaima, Boonchu, Tu, Changchun, Crameri, Gary, Stokes, Martha M., Hemachudha, Thiravat, Wang, Lin-Fa
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09.02.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 13/1; 13/106; 13/109; 45/23; 45/77; 45/88; 631/326/421; 631/326/596/4130; 82/80; ACE2; Amino Acid Sequence; Angiotensin-converting enzyme 2; Animals; Antibodies; Antibodies, Neutralizing - blood; Antisera; Asia, Southeastern; Chiroptera; Chiroptera - blood; Chiroptera - virology; Coronaviridae; Coronaviruses; COVID-19; COVID-19 - virology; Disease transmission; Gene sequencing; Genomes; Geographical distribution; Geography; Humanities and Social Sciences; multidisciplinary; Neutralization Tests; Pandemics; Pangolins - virology; Phylogeny; Protein Domains; Receptors, Cell Surface - chemistry; Receptors, Cell Surface - metabolism; Rhinolophus acuminatus; SARS-CoV-2 - physiology; Science; Science (multidisciplinary); Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Viral diseases; Viruses; Wildlife; Asia, Southeastern; Thailand; Southeast Asia; China
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-21240-1

Among the many questions unanswered for the COVID-19 pandemic are the origin of SARS-CoV-2 and the potential role of intermediate animal host(s) in the early animal-to-human transmission. The discovery of RaTG13 bat coronavirus in China suggested a high probability of a bat origin. Here we report molecular and serological evidence of SARS-CoV-2 related coronaviruses (SC2r-CoVs) actively circulating in bats in Southeast Asia. Whole genome sequences were obtained from five independent bats ( Rhinolophus acuminatus ) in a Thai cave yielding a single isolate (named RacCS203) which is most related to the RmYN02 isolate found in Rhinolophus malayanus in Yunnan, China. SARS-CoV-2 neutralizing antibodies were also detected in bats of the same colony and in a pangolin at a wildlife checkpoint in Southern Thailand. Antisera raised against the receptor binding domain (RBD) of RmYN02 was able to cross-neutralize SARS-CoV-2 despite the fact that the RBD of RacCS203 or RmYN02 failed to bind ACE2. Although the origin of the virus remains unresolved, our study extended the geographic distribution of genetically diverse SC2r-CoVs from Japan and China to Thailand over a 4800-km range. Cross-border surveillance is urgently needed to find the immediate progenitor virus of SARS-CoV-2. A bat origin for SARS-CoV-2 has been proposed. Here, by sampling wild Rhinolophus acuminatus bats from Thailand, the authors identified a SARS-CoV-2-related coronavirus (SC2r-CoV), designated as RacCS203, with 91.5% genome similarity to SARS-CoV-2, and show that sera obtained from bats and Malayan pangolin neutralize SARS-CoV-2.