c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae

• Published in: Nature communications Vol. 11; no. 1; pp. 1549 - 16
• Main Authors: Floyd, Kyle A., Lee, Calvin K., Xian, Wujing, Nametalla, Mahmoud, Valentine, Aneesa, Crair, Benjamin, Zhu, Shiwei, Hughes, Hannah Q., Chlebek, Jennifer L., Wu, Daniel C., Hwan Park, Jin, Farhat, Ali M., Lomba, Charles J., Ellison, Courtney K., Brun, Yves V., Campos-Gomez, Javier, Dalia, Ankur B., Liu, Jun, Biais, Nicolas, Wong, Gerard C. L., Yildiz, Fitnat H.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25.03.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 14/19; 14/28; 14/34; 631/326/41/88; 631/326/421; 631/326/46; 631/80/79; 82/111; 82/29; 82/58; Adenosine triphosphatase; Adenosine Triphosphatases - genetics; Adenosine Triphosphatases - metabolism; Anchoring; Attachment; Bacteria; Bacterial Adhesion; Biofilms; Biofilms - growth & development; Cell Tracking; Colonization; Cyclic GMP - analogs & derivatives; Cyclic GMP - metabolism; Fimbriae Proteins - genetics; Fimbriae Proteins - metabolism; Fimbriae, Bacterial - genetics; Fimbriae, Bacterial - metabolism; Hemagglutinins; Humanities and Social Sciences; Levels; Mannose; Movement; multidisciplinary; Science; Science (multidisciplinary); Vibrio cholerae; Vibrio cholerae - cytology; Vibrio cholerae - metabolism; Vibrio cholerae - physiology; Waterborne diseases
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-15331-8

Biofilm formation by Vibrio cholerae facilitates environmental persistence, and hyperinfectivity within the host. Biofilm formation is regulated by 3’,5’-cyclic diguanylate (c-di-GMP) and requires production of the type IV mannose-sensitive hemagglutinin (MSHA) pilus. Here, we show that the MSHA pilus is a dynamic extendable and retractable system, and its activity is directly controlled by c-di-GMP. The interaction between c-di-GMP and the ATPase MshE promotes pilus extension, whereas low levels of c-di-GMP correlate with enhanced retraction. Loss of retraction facilitated by the ATPase PilT increases near-surface roaming motility, and impairs initial surface attachment. However, prolonged retraction upon surface attachment results in reduced MSHA-mediated surface anchoring and increased levels of detachment. Our results indicate that c-di-GMP directly controls MshE activity, thus regulating MSHA pilus extension and retraction dynamics, and modulating V. cholerae surface attachment and colonization. Biofilm formation by Vibrio cholerae is regulated by c-di-GMP and requires the type IV MSHA pilus. Here, Floyd et al. show that the MSHA pilus is a dynamic system, and that both extension and retraction are directly controlled by c-di-GMP via regulation of activity of the extension ATPase MshE.