Tbx5a lineage tracing shows cardiomyocyte plasticity during zebrafish heart regeneration

• Published in: Nature communications Vol. 9; no. 1; pp. 428 - 13
• Main Authors: Sánchez-Iranzo, Héctor, Galardi-Castilla, María, Minguillón, Carolina, Sanz-Morejón, Andrés, González-Rosa, Juan Manuel, Felker, Anastasia, Ernst, Alexander, Guzmán-Martínez, Gabriela, Mosimann, Christian, Mercader, Nadia
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 30.01.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 13/2; 38/1; 38/32; 38/39; 38/91; 42; 59; 631/136/334/1874/763; 631/532/489; 64; 64/116; 692/4019/592/2725; 692/4019/592/2726; Animals; Animals, Genetically Modified; Cardiomyocytes; Cell Differentiation; Cell fate; Cell Lineage - genetics; Cell Tracking; Cortex; Danio rerio; Embryo, Nonmammalian; Gene Expression Regulation, Developmental; Genes, Reporter; Green Fluorescent Proteins - genetics; Green Fluorescent Proteins - metabolism; Heart; Heart diseases; Heart Ventricles - cytology; Heart Ventricles - growth & development; Heart Ventricles - metabolism; Humanities and Social Sciences; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; multidisciplinary; Myocardium; Myocardium - cytology; Myocardium - metabolism; Myocytes, Cardiac - cytology; Myocytes, Cardiac - metabolism; Myosin Light Chains - genetics; Myosin Light Chains - metabolism; Organogenesis - genetics; Plastic properties; Plasticity; Red Fluorescent Protein; Regeneration; Regeneration - genetics; Science; Science (multidisciplinary); Stem Cells - cytology; Stem Cells - metabolism; Superhigh frequencies; T-Box Domain Proteins - deficiency; T-Box Domain Proteins - genetics; Ventricle; Zebrafish; Zebrafish - genetics; Zebrafish - growth & development; Zebrafish - metabolism
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-017-02650-6

During development, mesodermal progenitors from the first heart field (FHF) form a primitive cardiac tube, to which progenitors from the second heart field (SHF) are added. The contribution of FHF and SHF progenitors to the adult zebrafish heart has not been studied to date. Here we find, using genetic tbx5a lineage tracing tools, that the ventricular myocardium in the adult zebrafish is mainly derived from tbx5a + cells, with a small contribution from tbx5a − SHF progenitors. Notably, ablation of ventricular tbx5a + -derived cardiomyocytes in the embryo is compensated by expansion of SHF-derived cells. In the adult, tbx5a expression is restricted to the trabeculae and excluded from the outer cortical layer. tbx5a -lineage tracing revealed that trabecular cardiomyocytes can switch their fate and differentiate into cortical myocardium during adult heart regeneration. We conclude that a high degree of cardiomyocyte cell fate plasticity contributes to efficient regeneration. It is not clear if it is the embryonic origin or anatomical location of cardiomyocytes that restrict their contribution to zebrafish heart regeneration. Here, the authors show a plasticity of embryonic precursors following tbx5a fate mapping and that trabecular cardiomyocytes help to rebuild the cortical myocardium.