Self-activating anti-infection implant

• Published in: Nature communications Vol. 12; no. 1; p. 6907
• Main Authors: Fu, Jieni, Zhu, Weidong, Liu, Xiangmei, Liang, Chunyong, Zheng, Yufeng, Li, Zhaoyang, Liang, Yanqin, Zheng, Dong, Zhu, Shengli, Cui, Zhenduo, Wu, Shuilin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 25.11.2021; Nature Publishing Group; Nature Portfolio
• Subjects: 140/146; 147/135; 147/143; 38; 38/77; 38/91; 631/326/22; 631/61/350/1057; 639/301/54/993; 639/766/119/544; 692/308/1426; Aerobic respiration; Anaerobic respiration; Animals; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Bacteria; Bacterial diseases; Bacterial infections; Biomedical materials; Bone and Bones - drug effects; Bone and Bones - pathology; Bone growth; Bone implants; Bone Regeneration - drug effects; Cell Differentiation; Cell Membrane; Cell membranes; Cell Proliferation - drug effects; Cell Respiration; Coating; Coatings; Communicable Diseases - drug therapy; Disease Models, Animal; Drug Implants; Durapatite; E coli; Electron transfer; Electron Transport; Escherichia coli; Escherichia coli Infections - drug therapy; Gene expression; Gene sequencing; Genes; Humanities and Social Sciences; Hydroxyapatite; Infections; Male; Mesenchymal Stem Cells; Mesenchyme; Metabolic pathways; Mitochondria; Molybdenum; Molybdenum disulfide; multidisciplinary; Osteoblastogenesis; Osteogenesis; Rats; Rats, Sprague-Dawley; Regeneration; Regeneration (physiology); Respiration; Science; Science (multidisciplinary); Staphylococcal Infections - drug therapy; Staphylococcus aureus; Staphylococcus infections; Stem cells; Surgical implants; Transplants & implants; Up-Regulation
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-27217-4

Clinically, it is difficult to endow implants with excellent osteogenic ability and antibacterial activity simultaneously. Herein, the self-activating implants modified with hydroxyapatite (HA)/MoS 2 coating are designed to prevent Staphylococcus aureus ( S. aureus ) and Escherichia coli ( E. coli ) infections and accelerate bone regeneration simultaneously. The electron transfer between bacteria and HA/MoS 2 is triggered when bacteria contacted with the material. RNA sequencing data reveals that the expression level of anaerobic respiration–related genes is up-regulated and the expression level of aerobic respiration–related genes is down-regulated when bacteria adhere to the implants. HA/MoS 2 presents a highly effective antibacterial efficacy against both S. aureus and E. coli because of bacterial respiration–activated metabolic pathway changes. Meanwhile, this coating promotes the osteoblastic differentiation of mesenchymal stem cells by altering the potentials of cell membrane and mitochondrial membrane. The proposed strategy exhibits great potential to endow implants with self-activating anti-infection performance and osteogenic ability simultaneously. Bone implants with antibacterial and osteogenic properties are important for clinical applications, but creating both properties simultaneously remains challenging. Here, the authors demonstrate a self-activating implant using a hydroxyapatite and molybdenum disulfide coating which accelerates bone regeneration and at the same time prevents bacterial infection.