Cryo-EM advances in RNA structure determination

Cryo-electron microscopy (cryo-EM) has emerged as an unprecedented tool to resolve protein structures at atomic resolution. Structural insights of biological samples not accessible by conventional X-ray crystallography and NMR can be explored with cryo-EM because measurements are carried out under n...

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Published inSignal transduction and targeted therapy Vol. 7; no. 1; pp. 58 - 6
Main Authors Ma, Haiyun, Jia, Xinyu, Zhang, Kaiming, Su, Zhaoming
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 23.02.2022
Nature Publishing Group
Subjects
631/337/2179
631/337/384
631/45/535
631/535
Cancer Research
Cell Biology
Cryoelectron Microscopy - methods
Crystallography
Electron microscopy
Internal Medicine
Medicine
Medicine & Public Health
NMR
Nuclear magnetic resonance
Oncology
Pathology
Perspective
Proteins
Ribonucleic acid
RNA
RNA - genetics
X-ray crystallography
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Summary:Cryo-electron microscopy (cryo-EM) has emerged as an unprecedented tool to resolve protein structures at atomic resolution. Structural insights of biological samples not accessible by conventional X-ray crystallography and NMR can be explored with cryo-EM because measurements are carried out under near-native crystal-free conditions, and large protein complexes with conformational and compositional heterogeneity are readily resolved. RNA has remained underexplored in cryo-EM, despite its essential role in various biological processes. This review highlights current challenges and recent progress in using cryo-EM single-particle analysis to determine protein-free RNA structures, enabled by improvement in sample preparation and integration of multiple structural and biochemical methods.
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ISSN:2059-3635
2095-9907
2059-3635
DOI:10.1038/s41392-022-00916-0