Quantifying white matter tract diffusion parameters in the presence of increased extra-fiber cellularity and vasogenic edema

• Published in: NeuroImage (Orlando, Fla.) Vol. 101; pp. 310 - 319
• Main Authors: Chiang, Chia-Wen, Wang, Yong, Sun, Peng, Lin, Tsen-Hsuan, Trinkaus, Kathryn, Cross, Anne H., Song, Sheng-Kwei
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2014; Elsevier Limited
• Subjects: Animals; Anisotropy; Brain; Computer Simulation; Diffusion; Diffusion basis spectrum imaging; Diffusion tensor imaging; Diffusion Tensor Imaging - methods; Edema - pathology; Experimental autoimmune encephalomyelitis (EAE); Female; Fourier transforms; Immunohistochemistry (IHC); Inflammation; Magnetic resonance imaging; Mice; Mice, Inbred C57BL; Monte Carlo Method; Monte-Carlo simulation; Multiple sclerosis; Multiple tensor model; Nerve Fibers, Myelinated - pathology; Neuritis, Autoimmune, Experimental - pathology; Optic Nerve - pathology; Phantoms, Imaging; Restricted diffusion; Trigeminal Nerve - pathology; White Matter - pathology; White matter injury; Experimental autoimmune encephalomyelitis (EAE); Multiple tensor model; Magnetic resonance imaging; Immunohistochemistry (IHC); Restricted diffusion; White matter injury; Inflammation; Monte-Carlo simulation; Diffusion tensor imaging; Diffusion basis spectrum imaging
• ISSN: 1053-8119; 1095-9572
• DOI: 10.1016/j.neuroimage.2014.06.064

The effect of extra-fiber structural and pathological components confounding diffusion tensor imaging (DTI) computation was quantitatively investigated using data generated by both Monte-Carlo simulations and tissue phantoms. Increased extent of vasogenic edema, by addition of various amount of gel to fixed normal mouse trigeminal nerves or by increasing non-restricted isotropic diffusion tensor components in Monte-Carlo simulations, significantly decreased fractional anisotropy (FA) and increased radial diffusivity, while less significantly increased axial diffusivity derived by DTI. Increased cellularity, mimicked by graded increase of the restricted isotropic diffusion tensor component in Monte-Carlo simulations, significantly decreased FA and axial diffusivity with limited impact on radial diffusivity derived by DTI. The MC simulation and tissue phantom data were also analyzed by the recently developed diffusion basis spectrum imaging (DBSI) to simultaneously distinguish and quantify the axon/myelin integrity and extra-fiber diffusion components. Results showed that increased cellularity or vasogenic edema did not affect the DBSI-derived fiber FA, axial or radial diffusivity. Importantly, the extent of extra-fiber cellularity and edema estimated by DBSI correlated with experimentally added gel and Monte-Carlo simulations. We also examined the feasibility of applying 25-direction diffusion encoding scheme for DBSI analysis on coherent white matter tracts. Results from both phantom experiments and simulations suggested that the 25-direction diffusion scheme provided comparable DBSI estimation of both fiber diffusion parameters and extra-fiber cellularity/edema extent as those by 99-direction scheme. An in vivo 25-direction DBSI analysis was performed on experimental autoimmune encephalomyelitis (EAE, an animal model of human multiple sclerosis) optic nerve as an example to examine the validity of derived DBSI parameters with post-imaging immunohistochemistry verification. Results support that in vivo DBSI using 25-direction diffusion scheme correctly reflect the underlying axonal injury, demyelination, and inflammation of optic nerves in EAE mice. •99- and 25-direction diffusion encoding schemes performed equally well in coherent white matter.•DBSI accurately assessed fiber directional diffusivity under conditions where DTI failed.•25-Direction DBSI of mouse optic nerve matched IHC detected pathologies.