Par complex cluster formation mediated by phase separation

• Published in: Nature communications Vol. 11; no. 1; pp. 2266 - 18
• Main Authors: Liu, Ziheng, Yang, Ying, Gu, Aihong, Xu, Jiawen, Mao, Ying, Lu, Haojie, Hu, Weiguo, Lei, Qun-Ying, Li, Zhouhua, Zhang, Mingjie, Cai, Yu, Wen, Wenyu
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.05.2020; Nature Publishing Group; Nature Portfolio
• Subjects: 14/19; 631/136/368; 631/45; 631/57; 631/80/641; 631/80/85; 64/24; 82/80; 82/83; Animals; Cell Cycle; Cell Differentiation; Cell Survival; Chlorocebus aethiops; Condensates; Condensation; Conformation; COS Cells; Drosophila; Drosophila melanogaster - cytology; Drosophila melanogaster - metabolism; Drosophila Proteins - chemistry; Drosophila Proteins - metabolism; Green Fluorescent Proteins - metabolism; HEK293 Cells; Humanities and Social Sciences; Humans; Insects; Larva - cytology; Liquid phases; multidisciplinary; Multiprotein Complexes - chemistry; Multiprotein Complexes - metabolism; Neuroblasts; Neurons - cytology; Neurons - metabolism; Oligomerization; Phase separation; Phosphorylation; Polarity; Protein Domains; Protein Kinase C - metabolism; Rats; Science; Science (multidisciplinary)
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-16135-6

The evolutionarily conserved Par3/Par6/aPKC complex regulates the polarity establishment of diverse cell types and distinct polarity-driven functions. However, how the Par complex is concentrated beneath the membrane to initiate cell polarization remains unclear. Here we show that the Par complex exhibits cell cycle-dependent condensation in Drosophila neuroblasts, driven by liquid–liquid phase separation. The open conformation of Par3 undergoes autonomous phase separation likely due to its NTD-mediated oligomerization. Par6, via C-terminal tail binding to Par3 PDZ3, can be enriched to Par3 condensates and in return dramatically promote Par3 phase separation. aPKC can also be concentrated to the Par3N/Par6 condensates as a client. Interestingly, activated aPKC can disperse the Par3/Par6 condensates via phosphorylation of Par3. Perturbations of Par3/Par6 phase separation impair the establishment of apical–basal polarity during neuroblast asymmetric divisions and lead to defective lineage development. We propose that phase separation may be a common mechanism for localized cortical condensation of cell polarity complexes. The evolutionarily conserved complex, the Par proteins, regulates cell polarity. Here, the authors show that in Drosophila neuroblasts, the Par complex exhibits liquid–liquid phase separation dependent on the cell cycle.