NIR-II nanoprobes in-vivo assembly to improve image-guided surgery for metastatic ovarian cancer

• Published in: Nature communications Vol. 9; no. 1; pp. 2898 - 10
• Main Authors: Wang, Peiyuan, Fan, Yong, Lu, Lingfei, Liu, Lu, Fan, Lingling, Zhao, Mengyao, Xie, Yang, Xu, Congjian, Zhang, Fan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 24.07.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 14/19; 14/33; 14/34; 14/63; 147/143; 631/1647/245; 631/61/350; 639/925; 692/699/67; Animals; Assembly; Cancer; Cell Line, Tumor; Deoxyribonucleic acid; DNA; Female; Fluorescence; Humanities and Social Sciences; Humans; Infrared windows; Medical imaging; Metastases; Metastasis; Mice; Microscopy, Electron, Transmission; multidisciplinary; Nanomaterials; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; Nanotechnology; Near infrared radiation; Neoplasm Metastasis; Ovarian cancer; Ovarian Neoplasms - pathology; Ovarian Neoplasms - surgery; Peptides; Prognosis; Reproducibility of Results; Science; Science (multidisciplinary); Solid tumors; Spectroscopy, Near-Infrared - methods; Surgery; Surgery, Computer-Assisted - methods; Tumors; Xenograft Model Antitumor Assays
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-018-05113-8

Local recurrence is a common cause of treatment failure for patients with solid tumors. Tumor-specific intraoperative fluorescence imaging may improve staging and debulking efforts in cytoreductive surgery and, thereby improve prognosis. Here, we report in vivo assembly of the second near-infrared window (NIR-II) emitting downconversion nanoparticles (DCNPs) modified with DNA and targeting peptides to improve the image-guided surgery for metastatic ovarian cancer. The NIR-II imaging quality with DCNPs is superior to that of clinically approved ICG with good photostability and deep tissue penetration (8 mm). Stable tumor retention period experienced 6 h by in vivo assembly of nanoprobes can be used for precise tumor resection. Superior tumor-to-normal tissue ratio is successfully achieved to facilitate the abdominal ovarian metastases surgical delineation. Metastases with ≤1 mm can be completely excised under NIR-II bioimaging guidance. This novel technology provides a general new basis for the future design of nanomaterials for medical applications. Real-time fluorescence imaging in the NIR-II region offers non-invasive detection of ovarian metastatic tumors and their image-guided surgery. Here the authors describe NIR-II nanoprobes in vivo assembly for detection of disseminated ovarian cancer.