Elucidating the genomic architecture of Asian EGFR-mutant lung adenocarcinoma through multi-region exome sequencing

• Published in: Nature communications Vol. 9; no. 1; pp. 216 - 11
• Main Authors: Nahar, Rahul, Zhai, Weiwei, Zhang, Tong, Takano, Angela, Khng, Alexis J., Lee, Yin Yeng, Liu, Xingliang, Lim, Chong Hee, Koh, Tina P. T., Aung, Zaw Win, Lim, Tony Kiat Hon, Veeravalli, Lavanya, Yuan, Ju, Teo, Audrey S. M., Chan, Cheryl X., Poh, Huay Mei, Chua, Ivan M. L., Liew, Audrey Ann, Lau, Dawn Ping Xi, Kwang, Xue Lin, Toh, Chee Keong, Lim, Wan-Teck, Lim, Bing, Tam, Wai Leong, Tan, Eng-Huat, Hillmer, Axel M., Tan, Daniel S. W.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.01.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 45; 45/22; 45/23; 631/67/1612/1350; 631/67/69; Adenocarcinoma; Adenocarcinoma - ethnology; Adenocarcinoma - genetics; Adenocarcinoma - pathology; Aneuploidy; Architecture; Asian Continental Ancestry Group - genetics; Cell cycle; Cell Line, Tumor; Comparative analysis; Drug resistance; Drug Resistance, Neoplasm - drug effects; Drug Resistance, Neoplasm - genetics; Epidermal growth factor receptors; ErbB Receptors - genetics; Genetic Predisposition to Disease - ethnology; Genetic Predisposition to Disease - genetics; Genomes; Genomic instability; Genomics - methods; Humanities and Social Sciences; Humans; Lung cancer; Lung Neoplasms - ethnology; Lung Neoplasms - genetics; Lung Neoplasms - pathology; Lungs; multidisciplinary; Mutation; Mutation rates; p53 Protein; Protein Kinase Inhibitors - pharmacology; Protein-tyrosine kinase; Science; Science (multidisciplinary); Smoking; Stability; Tumors; Tyrosine; Whole Exome Sequencing - methods
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-017-02584-z

EGFR -mutant lung adenocarcinomas (LUAD) display diverse clinical trajectories and are characterized by rapid but short-lived responses to EGFR tyrosine kinase inhibitors (TKIs). Through sequencing of 79 spatially distinct regions from 16 early stage tumors, we show that despite low mutation burdens, EGFR -mutant Asian LUADs unexpectedly exhibit a complex genomic landscape with frequent and early whole-genome doubling, aneuploidy, and high clonal diversity. Multiple truncal alterations, including TP53 mutations and loss of CDKN2A and RB1 , converge on cell cycle dysregulation, with late sector-specific high-amplitude amplifications and deletions that potentially beget drug resistant clones. We highlight the association between genomic architecture and clinical phenotypes, such as co-occurring truncal drivers and primary TKI resistance. Through comparative analysis with published smoking-related LUAD, we postulate that the high intra-tumor heterogeneity observed in Asian EGFR -mutant LUAD may be contributed by an early dominant driver, genomic instability, and low background mutation rates. EGFR mutant lung adenocarcinoma (LUAD) exhibit diverse clinical outcomes in response to targeted therapies. Here the authors show that these LUADs involve a complex genomic landscape with high intratumor heterogeneity, providing insights into the evolutionary trajectory of oncogene-driven LUAD and potential mediators of EGFR TKI resistance.