MIR22HG inhibits breast cancer progression by stabilizing LATS2 tumor suppressor

• Published in: Cell death & disease Vol. 12; no. 9; p. 810
• Main Authors: Deng, Xiaochong, Ye, Danrong, Hua, Kaiyao, Song, Hongming, Luo, Qifeng, Munankarmy, Amik, Liu, Diya, Zhou, Baian, Zheng, Wenfang, Zhou, Xiqian, Ji, Changle, Wang, Xuehui, Yu, Yunhe, Fang, Lin
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 26.08.2021; Springer Nature B.V; Nature Publishing Group
• Subjects: 13/1; 13/109; 13/89; 45/77; 45/90; 631/337/384/2568; 631/67/1347; 64/60; 82/80; Antibodies; Base Sequence; Biochemistry; Biomedical and Life Sciences; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - pathology; Cell Biology; Cell Culture; Cell Line, Tumor; Cell migration; Cell Movement - genetics; Cell proliferation; Cell Proliferation - genetics; Cytoplasm; Disease Progression; Down-Regulation - genetics; Female; Gain of Function Mutation - genetics; Gene Expression Regulation, Neoplastic; Humans; Immunology; Kinases; Life Sciences; MicroRNAs - genetics; MicroRNAs - metabolism; Models, Biological; Mutation - genetics; Phosphorylation; Protein Serine-Threonine Kinases - genetics; Protein Serine-Threonine Kinases - metabolism; Protein Stability; Transcriptomics; Tumor suppressor genes; Tumor Suppressor Proteins - genetics; Tumor Suppressor Proteins - metabolism; Yes-associated protein
• ISSN: 2041-4889; 2041-4889
• DOI: 10.1038/s41419-021-04105-9

The long noncoding RNA called MIR22 host gene (MIR22HG) was previously identified as a tumor suppressor in several cancers. However, the biological function of MIR22HG in breast cancer remains unknown. In this study, we aimed to determine the function and molecular mechanism of MIR22HG in breast cancer progression using transcriptomics and biotechnological techniques. Our results showed that MIR22HG expression was lower in the cancerous tissues than in the paired adjacent normal breast tissues. Additionally, MIR22HG was found to be mainly located in the cytoplasm and acted as a miR-629-5p sponge. Notably, MIR22HG stabilized the expression of large tumor suppressor 2 (LATS2), which promoted the LATS2-dependent phosphorylation of YAP1 and suppressed the expression of its downstream target oncogenes, thereby inhibiting the proliferation and migration of breast cancer cells. Therefore, our findings reveal the MIR22HG-dependent inhibition of breast cancer cell proliferation and migration via the miR-629-5p/LATS2 pathway, providing new insights and identifying novel therapeutic targets for breast cancer treatment.