A Turnip yellow mosaic virus infection system in Arabidopsis suspension cell culture

Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance w...

Full description

Saved in:
Bibliographic Details
Published inFEBS letters Vol. 581; no. 2; pp. 337 - 341
Main Authors Camborde, Laurent, Tournier, Vincent, Noizet, Mildred, Jupin, Isabelle
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 23.01.2007
Wiley
Subjects
Arabidopsis - cytology
Arabidopsis - drug effects
Arabidopsis - virology
Arabidopsis suspension culture
Arabidopsis thaliana
Bleomycin - pharmacology
BMV
Brome mosaic virus
Capsid Proteins - genetics
Cell Culture Techniques
Cell Line - virology
coat protein
Drug Resistance
Gene Expression
Genes, Reporter
GUS
hour post-transfection
hpt
Life Sciences
Microbiology and Parasitology
PAGE
PCR
Plant Diseases - virology
polyacrylamide gel electrophoresis
polymerase chain reaction
post-transcriptional gene silencing
PTGS
RdRp
Replication complex
Replicon
RNA-dependent RNA polymerase
Selectable replicon
subgenomic
Suspensions
Turnip yellow mosaic virus
Tymovirus - genetics
Tymovirus - physiology
TYMV
Virology
Virus Replication
wild-type
Zeocin
β-glucuronidase
Replication complex
GUS
Turnip yellow mosaic virus
TYMV
PAGE
BMV
CP
hpt
Selectable replicon
Arabidopsis suspension culture
Zeocin
sg
PTGS
RdRp
wt
PCR
Online AccessGet full text

Cover

More Information
Summary:Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance were used to transfect Arabidopsis cells. Zeocin-resistant Arabidopsis calli were used to generate a suspension cell culture. Detection of viral proteins and RNAs after 18 months in culture demonstrated persistent replication of the replicon. The Arabidopsis cell culture yielded soluble, active replication complexes, providing a useful tool to study host factors involved in TYMV replication.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2006.12.045