The emergence of the cortisol circadian rhythm in monozygotic and dizygotic twin infants: the twin-pair synchrony

• Published in: Clinical endocrinology (Oxford) Vol. 66; no. 2; pp. 192 - 197
• Main Authors: Custodio, Rodrigo Jose, Junior, Carlos Eduardo Martinelli, Milani, Soraya Lopes Sader, Simões, Aguinaldo Luis, De Castro, Margaret, Moreira, Ayrton Custodio
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.02.2007; Blackwell
• Subjects: Analysis of Variance; Biological and medical sciences; Child Development - physiology; Circadian Rhythm - genetics; Endocrinopathies; Female; Fundamental and applied biological sciences. Psychology; Humans; Hydrocortisone - analysis; Infant, Newborn; Linear Models; Longitudinal Studies; Medical sciences; Original; Saliva - chemistry; Twins, Dizygotic; Twins, Monozygotic; Vertebrates: endocrinology; Human; Dizygotic twin; Infant; Cortisol; Biological rhythm; Circadian rhythm; Monozygotic twin; Endocrinology
• ISSN: 0300-0664; 1365-2265
• DOI: 10.1111/j.1365-2265.2006.02706.x

Summary Objective  Studies on the influence of genetic factors on the ontogeny of cortisol circadian rhythm in infants are lacking. This study evaluated the influence of twinning and the heritability on the age of emergence of salivary cortisol rhythm. Design and subjects  A longitudinal study was performed using salivary samples obtained during morning and night, at 2, 4, 8, 12, 16, 20 and 24 weeks of postnatal life in 34 infants, 10 monozygotic (MZ) and 7 dizygotic (DZ) twin pairs. Salivary cortisol was determined by radioimmunoassay (RIA). Zigosity was verified by DNA analysis of at least 13 short tandem repeat polymorphisms. Difference of the emergence of cortisol circadian rhythm, within each twin pair, the intraclass correlation coefficient and the heritability index (h2) were calculated. Results  The mean (± SEM) age of emergence of salivary cortisol circadian rhythm was similar in MZ and DZ (7·8 ± 1·0 vs 7·4 ± 1·3 weeks). Seven pairs showed coincidence of the emergence of cortisol rhythm. Ten pairs were not coincident; among them the within‐pair difference of emergence of salivary circadian rhythm was similar in both MZ and DZ groups. The intraclass correlation coefficients were rMZ = 0·60, P = 0·02; and rDZ = 0·65, P = 0·03, respectively. The heritability index (h2) was 0·21 (ns). Conclusions  Salivary circadian rhythm appeared at the same postnatal age in MZ and DZ twin infants. Although several physiological aspects might be involved, the heritability index, obtained in the present study, suggests less genetic than environmental impact on the age of the onset of the cortisol circadian rhythm. Our data also indicated that each twin‐pair show synchrony because they probably shared prenatal and postnatal environmental synchronizers.