Wnt3a对结肠癌SW480细胞增殖的影响及作用机制

目的探讨Wnt3a对结肠癌SW480细胞增殖的调控效应及其作用机制。方法将培养好的结肠癌细胞SW480随机分为对照组和Wnt3a组,对照组仅给予DMEM培养基处理,Wnt3a组给予100 ng/m L Wnt3a培养。用MTT法检测细胞增殖情况,计算相对活细胞百分比;用平板克隆形成实验检测细胞克隆形成率（PE）;用流式细胞术检测细胞周期分布;用Western blotting法检测细胞内醛脱氢酶1B1（ALDH1B1）蛋白表达,RT-PCR技术检测ALDH1B1 mRNA表达。结果 Wnt3a诱导24、48、72 h,Wnt3a组相对活细胞百分比高于对照组（P均〈0.05）。Wnt3a诱导7...

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Bibliographic Details
Published in山东医药 Vol. 56; no. 37; pp. 18 - 20
Main Author 马松林 张姮 廖宇圣 徐丹 吴杰
Format Magazine Article
LanguageChinese
Published 华中科技大学同济医学院附属武汉市中心医院,武汉,430014 2016
Subjects
SW480细胞
Wnt3a
细胞增殖
结肠肿瘤
醛脱氢酶1B1
aldehyde dehydrogenase1B1
SW480细胞
colonic neoplasms
醛脱氢酶1B1
cell proliferation
SW480 cells
Wnt3a
结肠肿瘤
细胞增殖
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Summary:目的探讨Wnt3a对结肠癌SW480细胞增殖的调控效应及其作用机制。方法将培养好的结肠癌细胞SW480随机分为对照组和Wnt3a组,对照组仅给予DMEM培养基处理,Wnt3a组给予100 ng/m L Wnt3a培养。用MTT法检测细胞增殖情况,计算相对活细胞百分比;用平板克隆形成实验检测细胞克隆形成率（PE）;用流式细胞术检测细胞周期分布;用Western blotting法检测细胞内醛脱氢酶1B1（ALDH1B1）蛋白表达,RT-PCR技术检测ALDH1B1 mRNA表达。结果 Wnt3a诱导24、48、72 h,Wnt3a组相对活细胞百分比高于对照组（P均〈0.05）。Wnt3a诱导7 d,Wnt3a组PE高于对照组（P〈0.05）。Wnt3a诱导24 h,Wnt3a组G1期细胞百分比低于对照组,G2期细胞百分比高于对照组（P均〈0.05）。Wnt3a诱导48 h,Wnt3a组ALDH1B1蛋白、mRNA相对表达量高于对照组（P均〈0.05）。结论 Wnt3a可能通过诱导ALDH1B1高表达促进结肠癌SW480细胞增殖。
Bibliography:MA Songlin, ZHANG Heng, LIAO Yusheng, XU Dan, WU Jie （ Wuhan Central Hospital Affiliated to Huazhong University of Science and Technology, Wuhan 430014, China）
37-1156/R
colonic neoplasms; SW480 cells; Wnt3a; aldehyde dehydrogenase1B1; cell proliferation
Objective To investigate the potential role of Wnt3 a in the regulation of colon cancer cell proliferation and its regulatory mechanism. Methods Colon carcinoma cell line SW480 was randomly divided into two groups,the control group treated with DMEM,and the Wnt3 a group treated with 100 ng / m L Wnt3 a. The proliferation ability was measured by MTT. The relative percentage of living cells was also counted. Cell clone formation rate（ PE） was measured by plate clone formation assay. Cell cycle was analyzed by flow cytometry. RT-PCR was used to measure the expression level of ALDH1B1 mRNA. The expression level of aldehyde dehydrogenase（ ALDH） 1B1 protein was measured by Western blotting.Results After 24,48 and 72 h of induction by Wnt3 a,the relative percentage of
ISSN:1002-266X
DOI:10.3969/j.issn.1002-266X.2016.37.006