TILLING技术在作物突变研究中的应用现状与前景

定向诱导基因组局部突变（targeting induced local lesions in genomes,TILLING）技术将化学诱变与高通量突变检测技术相结合,可高效、快速地从突变群体中鉴定出目标基因突变位点。本文在概述TILLING技术应用于水稻、小麦、玉米、大豆等作物突变研究现状的基础上,重点综述了TILLING分析群体构建与突变位点检测方法的技术改进与发展,探讨了TILLING技术目前存在的问题与前景。...

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Published in植物遗传资源学报 Vol. 12; no. 4; pp. 581 - 587
Main Author 潘娜 郭会君 赵世荣 王广金 刘录祥
Format Journal Article
LanguageChinese
Published 中国农业科学院作物科学研究所/国家农作物航天诱变技术改良中心,北京,100081%黑龙江省农业科学院生物技术研究所,哈尔滨,150086 2011
Subjects
TILLING
作物
点突变
突变基因检测
进展
突变基因检测：进展
TILLING
点突变
作物
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ISSN1672-1810

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Summary:定向诱导基因组局部突变（targeting induced local lesions in genomes,TILLING）技术将化学诱变与高通量突变检测技术相结合,可高效、快速地从突变群体中鉴定出目标基因突变位点。本文在概述TILLING技术应用于水稻、小麦、玉米、大豆等作物突变研究现状的基础上,重点综述了TILLING分析群体构建与突变位点检测方法的技术改进与发展,探讨了TILLING技术目前存在的问题与前景。
Bibliography:PAN Na,GUO Hui-jun,ZHAO Shi-rong,WANG Guang-jin,LIU Lu-xiang（1National Center of Space Mutagenesis for Crop Improvement/Institute of Crop Science,Chinese Academy of Agricultural Sciences,Beijing 100081;2Biotechnology Research Institute,HeilongjiangAcademy of Agricultural Sciences,Harbin 150086）
11-4996/S
TILLING（targeting induced local lesions in genomes）is a complete reverse genetics approach for rapid and high throughput detecting of the target point mutations induced by chemical mutagen—Ethyl Methane Sulfonic acid（EMS）.This paper briefly introduced the application status of TILLING on main crops,i.e.,rice,wheat,corn and soybean,etc,with the emphasis on a review of the advances in methodology of mutagenized population construction and novel mutant alleles detect.The problems and future prospects of TILLING were also discussed.
Crop; TILLING; Point mutation; Novel mutant allele detection; Progress
ISSN:1672-1810