钼对小鼠后代醛氧化酶和黄嘌呤氧化酶转录水平的影响

试验选用60只二月龄的清洁级ICR小鼠,随机分成4组,每组10只雌性和5只雄性小白鼠,雌雄分开饲养,饮水中加入不同剂量的钼,分别是空白对照组、低钼组（100 mg.L-1）、中钼组（200 mg.L-1）、高钼组（400mg.L-1）,4周后雌雄合笼饲养。产仔后从每组仔鼠中随机雌性小白鼠30只和15只雄性小白鼠按原分组给予不同剂量的钼,8周后,再从每组中随机选取雌性小白鼠10只和5只雄性小白鼠合笼饲养,待其产仔。如此往复,使小鼠繁殖四代。以第三代（F2代）作为研究对象,应用实时荧光定量PCR的方法测定肝脏AOX和XO的转录水平。结果表明,与对照组相比F2代小鼠AOX和XO的转录水平随钼添加剂量...

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Published in东北农业大学学报 Vol. 43; no. 6; pp. 72 - 76
Main Author 张才 王雪莹 李鹏 白刃 陈庭锋 杨自军
Format Journal Article
LanguageChinese
Published 河南科技大学动物科技学院,河南洛阳,471003%沈阳农业大学畜牧兽医学院,沈阳,110161%沈阳沈北新区动物卫生管理局,沈阳,110121 2012
Subjects
小鼠
肝脏
醛氧化酶
钼
黄嘌呤氧化酶
黄嘌呤氧化酶
肝脏
小鼠
钼
醛氧化酶
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Summary:试验选用60只二月龄的清洁级ICR小鼠,随机分成4组,每组10只雌性和5只雄性小白鼠,雌雄分开饲养,饮水中加入不同剂量的钼,分别是空白对照组、低钼组（100 mg.L-1）、中钼组（200 mg.L-1）、高钼组（400mg.L-1）,4周后雌雄合笼饲养。产仔后从每组仔鼠中随机雌性小白鼠30只和15只雄性小白鼠按原分组给予不同剂量的钼,8周后,再从每组中随机选取雌性小白鼠10只和5只雄性小白鼠合笼饲养,待其产仔。如此往复,使小鼠繁殖四代。以第三代（F2代）作为研究对象,应用实时荧光定量PCR的方法测定肝脏AOX和XO的转录水平。结果表明,与对照组相比F2代小鼠AOX和XO的转录水平随钼添加剂量的增加呈递增趋势,实验组与对照组差异显著（P〈0.05）。可见长期钼暴露可提高小鼠后代AOX和XO的转录水平。
Bibliography:To study the effects of Mo on transcriptional level of aldehyde oxidase（AOX） and aldehyde oxidase（XO） in the progeny of animal, 60 2-month-old ICR mice（male： female=2：1）were selected in the test. After 5 days for acclimatization, they were divided into four groups with 10 female and 5 male in each group. Male and female mice were reared apart. Different concentrations of sodium molybdate were added in water and the mice were divided into control group, low Mo group（100 mg. L^-1）, mid-Mo group （200 mg- L^-1）and high Mo group（400 mg- L^-1）. Male and female were put in the same cage for rearing. After delivery,30 female and 15 male mice were selected from the newborn mice and were fed with Mo as the original groups. After 8 weeks, 10 female and 5 male mice were selected for rearing from the new groups. Littered size to the 4th generation. The 3th generation mice （F2 generation） were chosen as laboratory animals, and the liver were taken and detected for transcriptional level of AOX and XO by the method of fluore
ISSN:1005-9369
DOI:10.3969/j.issn.1005-9369.2012.06.016