Identification of HM13 as a prognostic indicator and a predictive biomarker for immunotherapy in hepatocellular carcinoma

• Published in: BMC cancer Vol. 22; no. 1; pp. 1 - 888
• Main Authors: Zhang, Genhao, Lv, Xianping, Yang, Qiankun, Liu, Hongchun
• Format: Journal Article
• Language: English
• Published: London BioMed Central Ltd 13.08.2022; BioMed Central; BMC
• Subjects: Biomarkers; Biopsy; Cancer therapies; Cell culture; Cell interactions; Cell migration; Cell proliferation; Cell signaling; Forecasts and trends; Gene expression; Genetic aspects; HCC; Hepatocellular carcinoma; Hepatoma; Histocompatibility; Histocompatibility antigens; HLA histocompatibility antigens; HM13; Identification and classification; Immune checkpoint inhibitors; Immunohistochemistry; Immunotherapy; Infections; Intracellular signalling; Liver cancer; Medical research; Metabolic syndrome; Metastases; Peptides; Prognosis; Protein expression; Proteins; Risk factors; Software; Statistical analysis; Therapeutic targets; Tumor microenvironment; Tumor-infiltrating lymphocytes; China; United States > US
• ISSN: 1471-2407; 1471-2407
• DOI: 10.1186/s12885-022-09987-2

Background Histocompatibility minor 13 (HM13) is a signal sequence stubbed intramembrane cleavage catalytic protein that is essential for cell signaling, intracellular communication, and cancer. However, the expression of HM13 and its prognostic value, association with tumor-infiltrating immune cells (TIICs) in the microenvironment, and potential to predict immunotherapeutic response in HCC are unknown. Methods The HM13 expression, clinicopathology analysis, and its influence on survival were analyzed in multiple public databases and further verified in collected HCC and normal tissues by qRT-PCR and immunohistochemistry staining assay (IHC). Furthermore, the lentivirus vector encoding HM13-shRNA to manipulate HM13 expression was selected to investigate whether HM13 could influence the malignant growth and metastasis potential of HCC cells. Finally, significant impacts of HM13 on the HCC tumor microenvironment (TME) and reaction to immune checkpoint inhibitors were analyzed. Results Upregulated HM13 was substantially correlated with poor prognosis in patients with HCC, and could facilitate the proliferation and migratory potential of HCC cells. Additionally, patients with high HM13 expression might be more sensitive to immunotherapy. Conclusions HM13 might be a prognostic biomarker and potential molecular therapeutic target for HCC. Keywords: HM13, Prognosis, Immune checkpoint inhibitors, HCC, Immunotherapy