Isolation and Characterization of a Novel Pathogenic Strain of Ehrlichia minasensis

• Published in: Microorganisms (Basel) Vol. 7; no. 11; p. 528
• Main Authors: Moura de Aguiar, Daniel, Pessoa Araújo Junior, João, Nakazato, Luciano, Bard, Emilie, Aguilar-Bultet, Lisandra, Vorimore, Fabien, Leonidovich Popov, Vsevolod, Moleta Colodel, Edson, Cabezas-Cruz, Alejandro
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 05.11.2019; MDPI
• Subjects: anaplasmataceae; Bacteria; bovine ehrlichiosis; Cattle; Cell culture; Clustering; Deoxyribonucleic acid; dh82; DNA; Ehrlichia; ehrlichia minasensis; Ehrlichiosis; Electron microscopy; Embryos; Epidemiology; Genes; genome; Genomes; Geographical distribution; Life Sciences; Pathogens; Phylogenetics; Phylogeny; Propagation; Subsystems; Ticks; Transmission electron microscopy; Veterinary medicine; Brazil; Canada; United States > US; Ehrlichia minasensis; DH82; bovine ehrlichiosis; genome; transmission electron microscopy; anaplasmataceae
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms7110528

The genus is composed of tick-borne obligate intracellular gram-negative alphaproteobacteria of the family Anaplasmataceae. includes important pathogens affecting canids ( , , and ), rodents ( ), and ruminants ( ). , an closely related to , was initially reported in Canada and Brazil. This bacterium has now been reported in Pakistan, Malaysia, China, Ethiopia, South Africa, and the Mediterranean island of Corsica, suggesting that has a wide geographical distribution. Previously, was found to cause clinical ehrlichiosis in an experimentally infected calf. The type strain UFMG-EV was successfully isolated from ticks and propagated in the tick embryonic cell line of (IDE8). However, the isolation and propagation of strains from cattle has remained elusive. In this study, the strain Cuiabá was isolated from an eight-month-old male calf of Holstein breed that was naturally infected with the bacterium. The calf presented clinical signs and hematological parameters of bovine ehrlichiosis. The in vitro culture of the agent was established in the canine cell line DH82. Ehrlichial morulae were observed using light and electron microscopy within DH82 cells. Total DNA was extracted, and the full genome of the strain Cuiabá was sequenced. A core-genome-based phylogenetic tree of spp. and spp. confirmed that is a sister taxa of . A comparison of functional categories among showed that has significantly less genes in the 'clustering-based subsystems' category, which includes functionally coupled genes for which the functional attributes are not well understood. Results strongly suggest that is a novel pathogen infecting cattle. The epidemiology of this deserves further attention because these bacteria could be an overlooked cause of tick-borne bovine ehrlichiosis, with a wide distribution.